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Chlorhexidine: An effective anticovid mouth rinse
Ashish Jain1, Vishakha Grover2, Charandeep Singh3
1Department of Periodontology, Dr. H. S. J. Institute of Dental Sciences, Panjab University, Chandigarh, India.
Journal of Indian Society of Periodontology
|March 1, 2021
Summary
Chlorhexidine digluconate (0.2%) demonstrated superior SARS-CoV-2 inactivation compared to povidone-iodine in laboratory tests. Both mouthwashes show antiviral activity, but chlorhexidine achieved over 99.9% inactivation rapidly.
Area of Science:
- Virology
- Microbiology
- Dentistry
Background:
- COVID-19 pandemic created uncertainty for dentists regarding viral load reduction.
- Conflicting evidence exists on mouthwash efficacy, particularly Chlorhexidine, against SARS-CoV-2 in aerosols and oral cavities.
Purpose of the Study:
- To comparatively evaluate the in-vitro antiviral efficacy of Chlorhexidine digluconate and Povidone Iodine against SARS-CoV-2.
- To assess the effectiveness of these agents as control measures in dental settings.
Main Methods:
- In-vitro laboratory analysis conducted in a BSL3 facility.
- Experiments utilized VeroE6 cell lines and SARS-CoV-2.
- Viral RNA quantification via Real-Time Quantitative Reverse Transcription PCR (qRT-PCR) determined virus inactivation.
Main Results:
- Chlorhexidine digluconate (0.2%) inactivated >99.9% of SARS-CoV-2 within 30 seconds.
- This efficacy surpassed that of povidone-iodine at 30 and 60 seconds.
- Both agents exhibited antiviral activity, with Chlorhexidine showing a greater relative change in Ct values.
Conclusions:
- Chlorhexidine digluconate (0.2%) effectively inactivates SARS-CoV-2 rapidly (30 seconds).
- Both Chlorhexidine and Povidone-Iodine demonstrate antiviral properties against SARS-CoV-2.
- Findings support the potential use of these mouthwashes in mitigating viral transmission in dental procedures.
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