A Clinical Study on Detection of Dysplastic Cells Through Saliva

Saliva is an ideal tool for liquid biopsy as it is noninvasive, easily collected, and easily transported. Saliva is used to detect various biomarkers in oral cancer patients, but none used for detecting salivary dysplastic cells in potentially malignant and malignant disorders. To detect dysplastic cells from saliva in oral cancer and potentially malignant patients and compare with wedge biopsy. All potentially malignant and malignant cases of oral cavity were included in this study. In these patients, unstimulated saliva was collected and centrifuged. The concentrated salivary substrate was stained by Haematoxylin and Eosin (H&E) stain, PAP (Papanicolaou) method and May Grunwald Giemsa stain (MGG) and analyzed. Wedge biopsy was performed from the representative area in the oral cavity and compared with salivary cells. In our study, out of 24 patients, 16 cases were malignant, and 8 were potentially malignant. 12 patients were found to be positive for atypical cells, 10 patients were found to be positive for dysplastic cells and atypical cells, and two patients were negative for atypical cells/dysplastic cells. In 10 patients who were positive for dysplastic cells, all were diagnosed histologically as malignancies (P = 0.001). The two patients who were negative for atypical cells/dysplastic cells showed precursor epithelial lesion with hyperplasia in the biopsy. Few neutrophils were found in 15 patients and increased in 9 patients (P = 0.039). Saliva is readily available, and can be collected noninvasively for detecting dysplastic cells.