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Factors affecting the rates at which nucleotides bind to Dowex 1 resin
1Department of Chemistry, Indiana University Bloomington 47405.
Analytical Biochemistry
|January 1, 1988
Abstract:
The relative rates at which adenine nucleotides bind to Dowex 1 anion-exchange resin are determined by diffusion through the immobile fluid film surrounding the resin particles and thus are unaffected by how tightly the nucleotides bind. By using a fine resin and stirring efficiently, the time needed for equilibration can be reduced from 4 h to less than 3 min.