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Updated: Nov 15, 2025

Purifying Plasmid DNA from Bacterial Colonies Using the Qiagen Miniprep Kit
Published on: July 29, 2007
Isolation of Chromosomal and Plasmid DNA from Bifidobacteria
Maria Esteban-Torres1, Lorena Ruiz2,3, Rocio Sanchez-Gallardo4
1School of Microbiology and APC Microbiome Ireland, University College Cork, Cork, Ireland. maria.estebantorres@ucc.ie.
Abstract:
Rapid and efficient protocols aimed at the isolation and purification of DNA for the purpose of downstream applications, such as cloning, PCR, Southern blotting, or sequencing, are essential for genetic, biochemical, and molecular biological analyses of a given bacterium. The protocols herein presented provide a robust and efficient method for the isolation of chromosomal and plasmid DNA from Bifidobacterium strains by organic extraction. The methods are simple, and the yield, purity, and quality of the DNA are adequate to perform various downstream applications including next-generation sequencing.
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