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Optimization of activated charcoal detoxification and concentration of chestnut shell hydrolysate for xylitol
Kubra Eryasar-Orer1, Seda Karasu-Yalcin2
1Department of Food Engineering, Faculty of Engineering, Bolu Abant Izzet Baysal University, Golkoy, Bolu, Turkey.
Objectives:
To increase xylose concentration of the chestnut shell hemicellulosic hydrolysate with an acceptable phenolic compound level in order to enhance xylitol production by Candida tropicalis M43.
Results:
The xylose concentration and total phenolic compound concentration of the hydrolysate were obtained as 33.68 g/L and 77.38 mg gallic acid equivalent/L, respectively by optimization of detoxification parameters and concentration level (60 °C, 115 min contact time, 5.942% (w/v) dosage of activated charcoal, 120 strokes/min shaking rate and 0.2 volume ratio). Xylitol production was achieved in the hydrolysate by using Candida tropicalis M43. The maximum xylitol concentration was 6.30 g/L and productivity, yield and percentage of substrate conversion were calculated as 0.11 g/L h, 19.13% and 97.79%, respectively. In addition, the chestnut shell hydrolysate fortified with xylose and the maximum xylitol concentration increased to 18.08 g/L in the hydrolysate-based medium containing 80 g/L xylose.
Conclusions:
Optimizing detoxification conditions with concentration level was found to be useful for enhancing xylitol production. In addition, fortification of the hydrolysate caused a three fold increase in maximum xylitol concentration.
