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Updated: Nov 15, 2025

Sequencing Small Non-coding RNA from Formalin-fixed Tissues and Serum-derived Exosomes from Castration-resistant Prostate Cancer Patients

Sequencing Small Non-coding RNA from Formalin-fixed Tissues and Serum-derived Exosomes from Castration-resistant Prostate Cancer Patients

Published on: November 19, 2019

Urinary Exosomes in Prostate Cancer.

Samanta Salvi¹, Erika Bandini², Francesco Fabbri²

¹Biosciences Laboratory, Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori (IRST) IRCCS, Meldola, Italy. samantasalvi@gmail.com.

Methods in Molecular Biology (Clifton, N.J.)

|March 2, 2021

Summary

This summary is machine-generated.

Urine analysis offers a less invasive method for prostate cancer biomarker discovery. This study details a workflow for characterizing extracellular vesicles (EVs) from urine, enhancing liquid biopsy potential.

Keywords:

MACSPlex kit Prostate cancer Urinary exosomes Urinary supernatant

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Published on: May 24, 2019

Area of Science:

Urology
Biochemistry
Oncology

Background:

Liquid biopsy is crucial for prostate cancer biomarker research.
Urine offers advantages over plasma/serum for less invasive analysis.
Extracellular vesicles (EVs) in urine are rich in potential biomarkers.

Purpose of the Study:

To develop and present a workflow for analyzing urinary extracellular vesicles (EVs).
To characterize the profile, concentration, and surface proteins of EVs from urinary supernatant.

Main Methods:

Workflow development for urinary EV isolation.
Analysis of EV protein profiles.
Quantification of EV concentration.
Characterization of EV surface proteins.

Main Results:

Established a workflow for urinary EV analysis.
Detailed the protein composition and concentration of urinary EVs.
Provided insights into EV surface characteristics.

Conclusions:

Urinary EVs represent a promising source for prostate cancer biomarkers.
The presented workflow facilitates comprehensive analysis of urinary EVs.
This approach enhances the potential of urine-based liquid biopsies.