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A new solution to the curved Ewald sphere problem for 3D image reconstruction in electron microscopy
J P J Chen1, K E Schmidt1, J C H Spence1
1Department of Physics, Arizona State University, Tempe, AZ 85287, USA.
Abstract:
We develop an algorithm capable of imaging a three-dimensional object given a collection of two-dimensional images of that object that are significantly influenced by the curvature of the Ewald sphere. These two-dimensional images cannot be approximated as projections of the object. Such an algorithm is useful in cryo-electron microscopy where larger samples, higher resolution, or lower energy electron beams are desired, all of which contribute to the significance of Ewald curvature.
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