Early transcriptome changes induced by the Geminivirus C4 oncoprotein: setting the stage for oncogenesis
Carl Michael Deom1, Magdy S Alabady2, Li Yang3
1Department of Plant Pathology, University of Georgia, Athens, GA, USA. deom@uga.edu.
BMC Genomics
|March 3, 2021
Summary
The Beet curly top virus C4 oncoprotein triggers early gene expression changes in plants, impacting hormone balance and cellular processes. This study reveals novel insights into viral pathogenesis and host-pathogen interactions.
Area of Science:
- Plant molecular biology
- Virology
- Genomics
Background:
- The Beet curly top virus (BCTV) C4 oncoprotein causes significant plant developmental issues.
- Previous research has not detailed the specific transcriptional changes induced by C4 expression.
Purpose of the Study:
- To investigate the early transcriptional effects of BCTV C4 oncoprotein expression in Arabidopsis.
- To understand the initial molecular events underlying C4-induced pathogenesis.
Main Methods:
- Comparative transcriptional analysis of messenger RNAs (mRNAs) and small RNAs.
- Analysis of gene expression at 6 and 12 hours post-induction.
Main Results:
- Identified 48 and 94 differentially expressed genes at 6- and 12-hours post-induction, respectively.
- C4-affected genes were categorized into those related to brassinosteroid (BR) signaling and those independent of BR signaling.
- Early changes impacted hormone homeostasis, cell development, cell wall integrity, redox balance, and lipid transport.
Conclusions:
- This study elucidates the initial genetic regulatory events triggered by the geminivirus C4 oncoprotein.
- The C4 protein influences multiple regulatory pathways, offering insights into its role in initiating pathogenesis.
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