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Immunogold Electron Microscopy

Immunogold Electron Microscopy

Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.

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Updated: Nov 15, 2025

Synthesis of Near-Infrared Emitting Gold Nanoclusters for Biological Applications

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Design and Development of Antibody Functionalized Gold Nanoparticles for Biomedical Applications.

José M Abad¹, Sara Puertas¹, Daniel Pérez¹

¹Nanoimmunotech, S.L. Edificio CITEXVI Fonte das Abelleiras s/n, Campus Universitario de Vigo, 36310 Vigo, Pontevedra, Spain.

Journal of Nanoscience and Nanotechnology

|March 3, 2021

Summary

This study presents an oriented antibody immobilization method for gold nanoparticles, enhancing biosystem development for biomedical applications. This technique ensures precise antibody orientation for improved antibody-antigen interactions.

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Area of Science:

Nanotechnology
Biomedical Engineering
Immunology

Background:

Antibody-functionalized gold nanoparticles offer a versatile interface biosystem.
Combining gold nanoparticle properties with antibody-antigen specificity is crucial for biomedical applications.

Purpose of the Study:

To provide insights into key factors for developing antibody-functionalized gold nanoparticles.
To develop an oriented antibody immobilization procedure on gold nanoparticles.

Main Methods:

Utilizing chelate-modified gold nanoparticles.
Designing for oriented immobilization of IgG antibodies (end-on spatial orientation).
Employing metal-chelation to the histidine-rich metal binding site in the Fc region of the antibody.

Main Results:

Successful oriented immobilization of IgG antibodies onto gold nanoparticles.
Demonstration of a specific metal-chelation strategy for antibody attachment.
Facilitation of end-on spatial orientation of antibodies.

Conclusions:

The developed oriented immobilization procedure is effective for antibody-functionalized gold nanoparticles.
This method enhances the development of advanced biosystems for biomedical applications.
Precise antibody orientation is achievable through targeted metal-chelation.