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Sulfatase Assay to Determine Influence of Plants on Microbial Activity in Soil

Anna Koprivova1, Achim Schmalenberger2, Stanislav Kopriva1

  • 1Botanical Institute and Cluster of Excellence on Plant Sciences (CEPLAS), University of Cologne, Cologne, Germany.

Bio-Protocol
|March 3, 2021
PubMed

Keywords:
ArabidopsisBacteriaMicrobiomeOrganic sulfurPlant-microbe interactionsSoilSulfatase

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Sulfur is an essential element in biological systems, contributing to synthesizing key biomolecules, including amino acids such as cysteine and methionine, and cofactors such as coenzyme A and biotin. Microorganisms primarily assimilate sulfur as sulfate (SO₄²⁻) from the environment, which must undergo a series of biochemical transformations before it can be incorporated into cellular components. As sulfate is highly oxidized, it must undergo assimilatory sulfate reduction to...
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