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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Related Experiment Video

Updated: Nov 15, 2025

Phospho Flow Cytometry with Fluorescent Cell Barcoding for Single Cell Signaling Analysis and Biomarker Discovery

Phospho Flow Cytometry with Fluorescent Cell Barcoding for Single Cell Signaling Analysis and Biomarker Discovery

Published on: October 4, 2018

Phospho-protein Analysis in Adherent Cells Using Flow Cytometry.

Renu Sharma¹, Amit Sharma¹, Atul Kumar¹

¹Stem Cells and Cancer Biology Group, Department of Biosciences and Bioengineering, Guwahati, India.

|March 3, 2021

Summary

This summary is machine-generated.

This study presents a flow cytometry method for quantifying intracellular protein phosphorylation at the single-cell level. This technique allows for detailed analysis of cell signaling pathways in heterogeneous cell populations.

Keywords:

Intracellular staining Phospho-protein Protein phosphorylation Signaling pathways Single cell analysis

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Flow Cytometric Analysis of Bimolecular Fluorescence Complementation: A High Throughput Quantitative Method to Study Protein-protein Interaction

Flow Cytometric Analysis of Bimolecular Fluorescence Complementation: A High Throughput Quantitative Method to Study Protein-protein Interaction

Published on: August 15, 2013

Area of Science:

Cellular Biology
Biochemistry
Immunology

Background:

Protein phosphorylation is a critical post-translational modification regulating protein activity.
Analyzing phosphorylation patterns provides insights into cellular responses to damage, cancer, and drugs.
Current methods like mass spectrometry quantify phosphorylation in bulk cell populations.

Purpose of the Study:

To develop a rapid, single-cell-based method for intracellular phospho-protein quantification.
To leverage flow cytometry for detailed analysis of differential signaling in heterogeneous cell populations.

Main Methods:

Utilized flow cytometry for intracellular phospho-protein detection.
Employed specific anti-phospho protein antibodies for targeted analysis.
Coupled phospho-protein data with cell surface marker expression.

Main Results:

Established a protocol for rapid, single-cell quantification of intracellular phospho-proteins.
Demonstrated the ability to analyze differential signaling within subpopulations.
Enabled simultaneous assessment of protein phosphorylation and cell surface markers.

Conclusions:

Flow cytometry offers a powerful tool for single-cell phospho-protein analysis.
This method enhances understanding of signaling in complex cellular environments.
The protocol facilitates rapid assessment of cellular states and drug responses.