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Magnetic-Activated Cell Sorting Using Coiled-Coil Peptides: An Alternative Strategy for Isolating Cells with High
Meng-Jie Shen1, René C L Olsthoorn1, Ye Zeng1
1Department of Supramolecular & Biomaterials Chemistry, Leiden Institute of Chemistry, Leiden University, Einsteinweg 55, Leiden 2333 CC, The Netherlands.
ACS Applied Materials & Interfaces
|March 3, 2021
Summary
A novel magnetic-activated cell sorting (MACS) method uses peptide interactions for cell separation without antigen presentation. This technique efficiently sorts cells and allows easy magnetic particle detachment, advancing cell separation research.
Area of Science:
- Biotechnology
- Cell Biology
- Biochemistry
Background:
- Magnetic-activated cell sorting (MACS) typically relies on cell surface antigen expression for separation.
- Existing MACS methods require specific cell surface markers for antibody-mediated magnetic particle binding.
Purpose of the Study:
- To develop an alternative MACS method utilizing coiled-coil peptide interactions.
- To enable cell sorting independent of endogenous antigen presentation.
- To facilitate efficient magnetic particle detachment post-sorting.
Main Methods:
- Cells (HeLa, CHO, NIH3T3) were engineered to incorporate or express coiled-coil peptides.
- Iron oxide particles were functionalized with complementary peptides.
- Co-incubation of peptide-labeled cells and functionalized particles enabled magnetic separation.
- Trypsin treatment was used for cell-particle detachment.
Main Results:
- Successful incorporation/expression of coiled-coil peptides in various cell lines.
- Efficient separation of labeled cells from unlabeled populations using functionalized magnetic particles.
- Demonstrated facile detachment of magnetic particles from sorted cells via trypsinization.
Conclusions:
- A novel, antigen-independent MACS method based on peptide interactions has been established.
- This method offers efficient cell sorting and simple magnetic particle removal.
- The system holds potential for applications in areas like separating genetically modified cells.
