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Express Arabidopsis Cryptochrome in Sf9 Insect Cells Using the Baculovirus Expression System
Xu Li1, Yawen Liu1, Hongtao Liu2
1National Key Laboratory of Plant Molecular Genetics (NKLPMG), CAS Center for Excellence in Molecular Plant Sciences, Shanghai Institutes for Biological Sciences (SIBS), Chinese Academy of Sciences, Shanghai, P. R. China.
Methods in Molecular Biology (Clifton, N.J.)
|March 3, 2021
Abstract:
The Bac-to-Bac® Baculovirus Expression System provides a rapid and efficient method to generate recombinant cryptochrome (CRY) proteins with chromophore flavin (FAD), which showed blue light response in vitro.
