Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

CODAvision: best practices and a user-friendly interface for rapid, customizable segmentation of medical images.

Nature protocols·2026
Same author

Quantitative 3D histology reveals localized immune remodeling during early pancreatic cancer progression.

Cell press blue·2026
Same author

3D multi-omics tumour atlases: from technology to biology and clinical translation.

Nature reviews. Cancer·2026
Same author

Particle tracking microrheology of cancer cells in living subjects.

Materials today (Kidlington, England)·2026
Same author

Distinct senescent β-cell senotypes differentially drive islet aging and dysfunction.

bioRxiv : the preprint server for biology·2026
Same author

Spatial Regulation of CAR Signaling Enables Logic-Gated Activity.

bioRxiv : the preprint server for biology·2026

Related Experiment Video

Updated: Nov 15, 2025

High-resolution Volume Imaging of Neurons by the Use of Fluorescence eXclusion Method and Dedicated Microfluidic Devices
09:11

High-resolution Volume Imaging of Neurons by the Use of Fluorescence eXclusion Method and Dedicated Microfluidic Devices

Published on: March 26, 2018

7.3K

Single Cell Volume Measurement Utilizing the Fluorescence Exclusion Method (FXm).

Nash D Rochman1, Kai Yao2, Nicolas Perez A Gonzalez1

  • 1Department of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, Maryland 21218, USA.

Bio-Protocol
|March 4, 2021
PubMed
Summary
This summary is machine-generated.

Measuring single cell size is crucial for understanding cell growth, tissue health, and diseases. This study details the Fluorescence Exclusion Method (FXm) protocol, simplifying cell volume analysis for broader research applications.

Keywords:
Cell mechanicsEpifluorescence microscopyImage analysisMicrofluidicsNeural networksSingle cell

More Related Videos

Techniques for the Analysis of Extracellular Vesicles Using Flow Cytometry
09:39

Techniques for the Analysis of Extracellular Vesicles Using Flow Cytometry

Published on: March 17, 2015

23.6K
Spot Variation Fluorescence Correlation Spectroscopy for Analysis of Molecular Diffusion at the Plasma Membrane of Living Cells
05:56

Spot Variation Fluorescence Correlation Spectroscopy for Analysis of Molecular Diffusion at the Plasma Membrane of Living Cells

Published on: November 12, 2020

3.0K

Related Experiment Videos

Last Updated: Nov 15, 2025

High-resolution Volume Imaging of Neurons by the Use of Fluorescence eXclusion Method and Dedicated Microfluidic Devices
09:11

High-resolution Volume Imaging of Neurons by the Use of Fluorescence eXclusion Method and Dedicated Microfluidic Devices

Published on: March 26, 2018

7.3K
Techniques for the Analysis of Extracellular Vesicles Using Flow Cytometry
09:39

Techniques for the Analysis of Extracellular Vesicles Using Flow Cytometry

Published on: March 17, 2015

23.6K
Spot Variation Fluorescence Correlation Spectroscopy for Analysis of Molecular Diffusion at the Plasma Membrane of Living Cells
05:56

Spot Variation Fluorescence Correlation Spectroscopy for Analysis of Molecular Diffusion at the Plasma Membrane of Living Cells

Published on: November 12, 2020

3.0K

Area of Science:

  • Cell Biology
  • Biophysics
  • Pathology

Background:

  • Accurate single cell size measurement is vital for understanding cell growth control, tissue homeostasis, organogenesis, and various pathologies.
  • Cell volume plays a critical role in regulating fundamental cell signaling pathways, including cell cycle progression.

Purpose of the Study:

  • To provide a detailed protocol for the Fluorescence Exclusion Method (FXm) for measuring single cell size.
  • To highlight the importance of cell volume in biological processes and disease.
  • To outline future advancements in image analysis for cell volume studies.

Main Methods:

  • The Fluorescence Exclusion Method (FXm) protocol is presented.
  • References to the historical development of the FXm are included.
  • Future directions in image analysis for FXm are discussed.

Main Results:

  • The FXm protocol is detailed for accurate cell volume measurement.
  • The study emphasizes the significance of cell size in cell cycle regulation.
  • Potential for expanded applications and accessibility of FXm is suggested.

Conclusions:

  • The Fluorescence Exclusion Method (FXm) offers a valuable tool for single cell size measurement.
  • Advancements in image analysis will enhance the utility and accessibility of FXm.
  • This method supports research in cell growth, homeostasis, and disease pathology.