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Related Concept Videos

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

968
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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CRISPR01:59

CRISPR

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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Related Experiment Video

Updated: Nov 15, 2025

CRISPR-Cas9-based Genome Engineering to Generate Jurkat Reporter Models for HIV-1 Infection with Selected Proviral Integration Sites
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HIV-CRISPR: A CRISPR/Cas9 Screening Method to Identify Genes Affecting HIV Replication.

Ferdinand Roesch1,2, Molly OhAinle1

  • 1Human Biology Division, Fred Hutchinson Cancer Research Center, Seattle, USA.

Bio-Protocol
|March 4, 2021
PubMed
Summary

This study presents a new CRISPR screening method for efficiently studying the entire HIV-1 life cycle. This approach enhances viral research by enabling high-throughput analysis of viral mechanisms.

Keywords:
CRISPR screenCofactorsHIVHIV-CRISPRHigh-Throughput Sequencing (HTS)Restriction factor

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Area of Science:

  • Virology
  • Molecular Biology
  • Genetics

Background:

  • CRISPR/Cas9 technology has revolutionized biological research, particularly in cancer, cell biology, and virology.
  • Its high throughput and low false discovery rates make it ideal for screening studies, including whole-genome screens.
  • Studying the complete human immunodeficiency virus type 1 (HIV-1) life cycle is crucial for developing effective antiviral strategies.

Purpose of the Study:

  • To develop and describe an efficient CRISPR screening protocol for comprehensive analysis of the HIV-1 life cycle.
  • To enable high-throughput screening of viral mechanisms throughout all stages of HIV-1 infection.

Main Methods:

  • A novel CRISPR screening protocol was developed.
  • The method involves packaging HIV-CRISPR lentiviral genomes.
  • This is achieved by infecting HIV-1 virus 'in trans'.

Main Results:

  • The described protocol allows for efficient screening of the entire HIV-1 life cycle.
  • This method facilitates high-throughput analysis of viral gene function and interactions.

Conclusions:

  • This CRISPR screening approach offers a powerful tool for advancing HIV-1 research.
  • It enables detailed investigation of viral replication and pathogenesis.
  • The protocol is adaptable for studying other viral systems.