Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Aging01:26

Aging

422
Aging is a complex biological phenomenon influenced by various processes that affect cellular and systemic functions. Several prominent theories attempt to explain its mechanisms, highlighting cellular limitations, oxidative damage, and hormonal changes as central factors in aging.
Cellular Clock Theory
The cellular clock theory posits that the human lifespan is closely tied to the finite capacity of cells to divide, a phenomenon governed by telomeres, which are protective caps at the ends of...
422
Menopause01:28

Menopause

3.3K
Menopause, a natural biological process marking the end of a woman's fertility, typically occurs between the fifth and sixth decade of life. This phase is characterized by the exhaustion of the ovarian follicle pool, leading to less responsive ovaries despite the high levels of Follicle Stimulating Hormone (FSH) and Luteinizing Hormone (LH). The consequential decrease in estrogen production results in symptoms like hot flashes, heavy sweating, headaches, hair loss, muscle pains, vaginal...
3.3K
The Effect of Aging on Tissues01:19

The Effect of Aging on Tissues

3.0K
Several body functions deteriorate with age. The external signs of aging are easily identifiable. For example, the skin becomes dry, less elastic, and thins out, forming wrinkles. The skin of the face begins to appear looser due to a decrease in the levels of elastic and collagen fibers in the connective tissue. Additionally, melanin production in the hair follicle decreases with age, resulting in gray hair. Moreover, the senses of sight and hearing decline, so glasses and hearing aids may...
3.0K
Spermatogenesis01:41

Spermatogenesis

107.1K
Spermatogenesis is the process by which haploid sperm cells are produced in the male testes. It starts with stem cells located close to the outer rim of seminiferous tubules. These spermatogonial stem cells divide asymmetrically to give rise to additional stem cells (meaning that these structures “self-renew”), as well as sperm progenitors, called spermatocytes. Importantly, this method of asymmetric mitotic division maintains a population of spermatogonial stem cells in the male...
107.1K
Oogenesis02:07

Oogenesis

67.5K
In human women, oogenesis produces one mature egg cell or ovum for every precursor cell that enters meiosis. This process differs in two unique ways from the equivalent procedure of spermatogenesis in males. First, meiotic divisions during oogenesis are asymmetric, meaning that a large oocyte (containing most of the cytoplasm) and minor polar body are produced as a result of meiosis I, and again following meiosis II. Since only oocytes will go on to form embryos if fertilized, this unequal...
67.5K
Radical Autoxidation01:20

Radical Autoxidation

2.7K
The oxidation of an organic compound in the presence of air or oxygen is called autoxidation. For example, cumene reacts with oxygen to form hydroperoxide. Autoxidation involves initiation, propagation, and termination steps. Many organic compounds are susceptible to autoxidation—especially ethers in the presence of oxygen, which form hydroperoxides. Even though this reaction is slow, old ether bottles contain small amounts of peroxide, which leads to laboratory explosions during ether...
2.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The Three Pillars of ATP Production in Mammalian Sperm: Integrating Gluconeogenesis Into the Metabolic Framework.

Reproductive medicine and biology·2026
Same author

Pregnancy via Testicular Sperm Extraction for Anejaculation After Neuroblastoma Surgery.

IJU case reports·2025
Same author

Natural pregnancy following kidney transplantation in an azoospermic male: a case report.

Translational andrology and urology·2025
Same author

Summary of the Clinical Practice Guidelines for Male Infertility by the Japanese Urological Association With the Support of the Japan Society for Reproductive Medicine.

International journal of urology : official journal of the Japanese Urological Association·2025
Same author

Successful sperm retrieval by microdissection testicular sperm extraction in a man with partial AZFb deletion: a case report.

Translational andrology and urology·2025
Same author

<i>Sod1</i> deficiency in mouse oocytes during in vitro maturation increases chromosome segregation errors with a reduced BUBR1 at kinetochore.

Reproductive medicine and biology·2025

Related Experiment Video

Updated: Nov 15, 2025

Evaluation of Intracellular Location of Reactive Oxygen Species in Solea Senegalensis Spermatozoa
10:24

Evaluation of Intracellular Location of Reactive Oxygen Species in Solea Senegalensis Spermatozoa

Published on: March 11, 2018

6.5K

Aging increases oxidative stress in semen.

Mitsuru Nago1, Akane Arichi2, Naoki Omura2

  • 1Denentoshi Ladies Clinic, Yokohama, Kanagawa, Japan. nago@art.email.ne.jp.

Investigative and Clinical Urology
|March 4, 2021
PubMed
Summary

Static oxidation-reduction potential (sORP) increases with paternal age, correlating with decreased sperm quality. Men over 34 showed a higher rate of positive sORP, indicating increased semen oxidative stress.

Keywords:
AgingOxidation reductionOxidative stressSemenSperm

More Related Videos

Analysis of Oxidative Stress in Zebrafish Embryos
11:05

Analysis of Oxidative Stress in Zebrafish Embryos

Published on: July 7, 2014

37.7K
Author Spotlight: A Simple and Cost-Effective Method for Leukocyte Analysis in Semen
04:36

Author Spotlight: A Simple and Cost-Effective Method for Leukocyte Analysis in Semen

Published on: January 19, 2024

4.9K

Related Experiment Videos

Last Updated: Nov 15, 2025

Evaluation of Intracellular Location of Reactive Oxygen Species in Solea Senegalensis Spermatozoa
10:24

Evaluation of Intracellular Location of Reactive Oxygen Species in Solea Senegalensis Spermatozoa

Published on: March 11, 2018

6.5K
Analysis of Oxidative Stress in Zebrafish Embryos
11:05

Analysis of Oxidative Stress in Zebrafish Embryos

Published on: July 7, 2014

37.7K
Author Spotlight: A Simple and Cost-Effective Method for Leukocyte Analysis in Semen
04:36

Author Spotlight: A Simple and Cost-Effective Method for Leukocyte Analysis in Semen

Published on: January 19, 2024

4.9K

Area of Science:

  • Reproductive biology
  • Oxidative stress markers
  • Male fertility

Background:

  • Aging is associated with increased oxidative stress, reduced sperm motility, and DNA fragmentation.
  • Previous studies on age-related semen changes focused on reactive oxygen species (ROS) and total antioxidant capacity (TAC).
  • A comprehensive evaluation of oxidative stress requires considering multiple parameters beyond ROS and TAC.

Purpose of the Study:

  • To investigate the relationship between static oxidation-reduction potential (sORP) and paternal age.
  • To evaluate sORP as a potential indicator of oxidative stress in semen.
  • To understand the impact of age on semen parameters and sORP levels.

Main Methods:

  • Analysis of semen samples from 173 men for volume, motility, and beat cross frequency (BCF).
  • Measurement of sORP using the MiOXSYS™ system.
  • Correlation analysis of semen parameters and sORP with age, and comparison of sORP positivity rates between age groups (<34 and ≥34 years).

Main Results:

  • Semen volume, motility, and BCF showed negative correlations with age (p<0.001).
  • Semen sORP levels demonstrated a positive correlation with age (p<0.05).
  • The rate of sORP positivity was significantly higher in men aged ≥34 years (33%) compared to those <34 years (12%; p<0.01).

Conclusions:

  • sORP may contribute to age-related declines in semen parameters like volume, motility, and BCF.
  • A significant increase in sORP positivity was observed in men over 34 years of age.
  • sORP serves as a valuable indicator for assessing age-related oxidative stress in semen.