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DNAJC5 promotes hepatocellular carcinoma cells proliferation though regulating SKP2 mediated p27 degradation

Hailong Wang1, Jiayu Luo1, Xuesi Tian1

  • 1Center for Experimental Medicine, the First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, China; Jiangxi Key Laboratory of Molecular Diagnostics and Precision Medicine, Nanchang, Jiangxi, China.

Biochimica Et Biophysica Acta. Molecular Cell Research
|March 4, 2021
PubMed

Insights

DNAJC5 protein promotes hepatocellular carcinoma (HCC) growth by interacting with SKP2 to degrade p27. This discovery reveals a new oncogenic mechanism for DNAJC5 in HCC, impacting patient prognosis.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cell Biology

Background:

  • DNAJC5 (DnaJ heat shock protein family member C5), also known as cysteine tandem protein (CSPα), is crucial for nerve tissue function.
  • The oncogenic role of DNAJC5 in cancer, particularly hepatocellular carcinoma (HCC), has not been previously established.

Purpose of the Study:

  • To investigate the potential oncogenic function of DNAJC5 in hepatocellular carcinoma (HCC).
  • To elucidate the molecular mechanism by which DNAJC5 contributes to HCC development and progression.

Main Methods:

  • Analysis of DNAJC5 protein expression in human HCC tissues.
  • Assessment of DNAJC5's effect on HCC cell proliferation and cell cycle.
  • Investigation of the interaction between DNAJC5, SKP2, and p27 using molecular assays.

Main Results:

  • DNAJC5 protein expression is elevated in HCC tissues and correlates with poor patient prognosis.
  • Overexpression of DNAJC5 enhances HCC cell proliferation by promoting cell cycle progression from G1 phase.
  • DNAJC5 interacts with SKP2, facilitating the degradation of p27 (a cyclin-dependent kinase inhibitor) and leading to decreased p27 protein levels.

Conclusions:

  • The DNAJC5-SKP2-p27 signaling pathway represents a novel mechanism for DNAJC5's oncogenic activity in HCC.
  • Targeting the DNAJC5-SKP2-p27 pathway may offer a therapeutic strategy for hepatocellular carcinoma.
Keywords:
Cell proliferationDNAJC5HCCSKP2p27

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