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Related Experiment Videos

Gene mapping with recombinant inbreds in maize.

B Burr1, F A Burr, K H Thompson

  • 1Biology Department, Brookhaven National Laboratory, Upton, New York 11973.

Genetics
|March 1, 1988
PubMed
Summary

New recombinant inbred lines (RILs) in maize facilitate rapid gene mapping. These RILs, derived from specific crosses, enable efficient chromosomal localization of molecular markers using a comprehensive genetic map.

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Area of Science:

  • Plant genetics
  • Genomics
  • Molecular biology

Background:

  • Developing efficient methods for gene mapping in maize is crucial for genetic research.
  • Recombinant inbred lines (RILs) offer a powerful tool for genetic analysis.
  • Previous mapping efforts in maize have established a foundation for new marker integration.

Purpose of the Study:

  • To develop and utilize maize recombinant inbred lines for rapid molecular probe mapping.
  • To create a comprehensive genetic map covering the maize genome.
  • To establish an efficient system for mapping new genes and molecular markers.

Main Methods:

  • Construction of two recombinant inbred families from F2 populations (T232 X CM37 and CO159 X Tx303).
  • Development of a genetic map utilizing isozymes and restriction fragment length polymorphisms (RFLPs).

Related Experiment Videos

  • Determination of allelic distributions of new genes within the RILs.

    • Main Results:

    • Successfully developed maize recombinant inbred lines.
    • Generated a genetic map encompassing nearly the entire maize genome.
    • Demonstrated the utility of RILs for comparative allelic distribution analysis.

    Conclusions:

    • Maize RILs provide an efficient platform for mapping molecular probes to specific chromosomal locations.
    • The developed genetic map and RILs streamline the process of integrating new genetic information.
    • This resource significantly enhances the ability to map novel molecular markers in maize.