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Ubiquitin-Modified Proteome of SARS-CoV-2-Infected Host Cells Reveals Insights into Virus-Host Interaction and

Huan Zhang1, Huanying Zheng2, Jinying Zhu1

  • 1Key Laboratory of Zoonotic of Liaoning Province, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, Liaoning Province, P. R. China.

Journal of Proteome Research
|March 5, 2021
PubMed

Insights

This study reveals how ubiquitination changes in host cells and SARS-CoV-2 proteins during COVID-19 infection. These findings offer new insights into virus-host interactions and potential therapeutic targets for COVID-19.

Area of Science:

  • Biochemistry
  • Virology
  • Immunology

Background:

  • The COVID-19 pandemic, caused by SARS-CoV-2, presents a significant global health challenge.
  • Understanding the pathogenesis and host immune response to SARS-CoV-2 is crucial but remains incomplete.

Purpose of the Study:

  • To investigate ubiquitination alterations in host cells and SARS-CoV-2 during infection.
  • To identify potential therapeutic targets for COVID-19 by examining virus-host interactions.

Main Methods:

  • Quantitative proteomic analysis using label-free liquid chromatography-tandem mass spectrometry.
  • Identification and quantification of lysine ubiquitination sites in host proteins and viral proteins.

Main Results:

  • Identified 8943 ubiquitination sites on 3086 proteins; 138 sites (104 proteins) upregulated and 828 sites (447 proteins) downregulated at 72h post-infection.
  • Bioinformatics analysis indicated SARS-CoV-2 modulates host immunity via ubiquitination of key proteins (USP5, IQGAP1, TRIM28, Hsp90).
  • Observed ubiquitination on 11 SARS-CoV-2 proteins involved in replication and immune evasion.

Conclusions:

  • SARS-CoV-2 infection significantly alters host cell ubiquitination patterns.
  • Ubiquitination of viral proteins suggests a role in virus replication and host immune response modulation.
  • This research provides novel insights into SARS-CoV-2-host interactions and potential therapeutic strategies for COVID-19.
Keywords:
LC-MScoronavirus disease 2019severe acute respiratory syndrome coronavirus 2ubiquitome

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