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Upconversion Nanoparticles Decorated with Polysialic Acid for Solid Tumors Visualization In Vivo.

P A Demina1,2, N V Sholina3,4, R A Akasov5,3,4

  • 1Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia. polidemina1207@yandex.ru.

Doklady. Biochemistry and Biophysics
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PubMed
Summary

This study biofunctionalized upconversion nanoparticles (UCNPs) with polysialic acid for enhanced in vivo imaging. This novel approach improves tumor visualization by prolonging circulation time and reducing non-specific uptake.

Keywords:
bioimagingpolysialic acidsurface functionalizationupconversion nanophosphors

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Area of Science:

  • Biomedical Engineering
  • Nanotechnology
  • Medical Imaging

Background:

  • Upconversion nanoparticles (UCNPs) offer deep tissue penetration for in vivo imaging.
  • UCNP surface functionalization is crucial for solid tumor visualization, requiring long circulation, biocompatibility, and non-toxicity.
  • Tumor accumulation depends on vascular architecture and lymphatic drainage.

Purpose of the Study:

  • To develop a biofunctionalization strategy for UCNPs using endogenous polysialic acid.
  • To create novel bioreagents for enhanced in vivo imaging of solid tumors.
  • To improve UCNP circulation time and reduce non-specific uptake for better tumor targeting.

Main Methods:

  • Biofunctionalization of UCNPs with polysialic acid.
  • Assessment of protein adsorption and macrophage uptake.
  • Evaluation of circulation time in vivo.
  • Monitoring of photoluminescent signal intensity in solid tumors.

Main Results:

  • Polysialic acid biofunctionalization significantly reduced non-specific protein adsorption and macrophage uptake.
  • UCNP bioreagents demonstrated prolonged circulation time in the bloodstream (up to 3 hours).
  • Intense photoluminescent signals were observed in solid tumors, indicating effective accumulation.

Conclusions:

  • Endogenous polysialic acid is an effective biofunctionalization agent for UCNPs.
  • This approach enhances UCNP performance for in vivo tumor imaging by improving pharmacokinetics.
  • The developed UCNP bioreagents show significant potential for advanced medical diagnostics.