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Related Concept Videos

Chromatography: Introduction01:10

Chromatography: Introduction

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Chromatography is a technique used to separate compounds based on differences of partitioning between two phases, the stationary phase and the mobile phase.
The phase in which the compounds linger or on which the compounds adsorb is called the stationary phase, whereas the mobile phase is the solvent that carries the solutes to be analyzed. In traditional column chromatography, the mixture flows through the stationary phase, and the compounds partition between the stationary and mobile phases...
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Principles Of Column Chromatography01:13

Principles Of Column Chromatography

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The chromatography technique was first invented in 1901 by Michael S. Tswett, a Russian botanist, to separate plant pigments using organic solvents. Further, in 1941, Archer John Porter Martin and R. L. M. Synge modified the technique by packing silica gel into a column. A mixture of amino acids was then separated on the packed column using chloroform and water mixture as the mobile phase. This was the first report on column chromatography. At present, column chromatography is a widely used...
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Chirality in Nature02:30

Chirality in Nature

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Chirality is the most intriguing yet essential facet of nature, governing life’s biochemical processes and precision. It can be observed from a snail shell pattern in a macroscopic world to an amino acid, the minutest building block of life. Most of the snails around the world have right-coiled shells because of the intrinsic chirality in their genes. All the amino acids present in the human body exist in an enantiomerically pure state, except for glycine - the sole achiral amino acid.
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Chromatographic Methods: Classification01:12

Chromatographic Methods: Classification

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Chromatographic techniques are classified in three ways: the classification is based on the physical state of the stationary and mobile phases, how the mobile phase and the stationary phase contact each other, or through the chemical or physical processes that isolate the components of the sample. Typically, the mobile phase is either a liquid or gas, while the stationary phase is either a solid or a liquid layer applied to a solid surface.
Chromatographic techniques are typically named by...
3.0K
Ion-Exchange Chromatography01:09

Ion-Exchange Chromatography

1.2K
Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...
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Affinity Chromatography01:03

Affinity Chromatography

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Affinity chromatography is a powerful technique extensively utilized for separating and purifying specific biomolecules from complex mixtures. It capitalizes on the highly selective binding between an analyte and its counterpart, such as antibody-antigen interactions. The counterpart is immobilized on the stationary phase, forming an affinity column. The stationary phase typically consists of solid support, such as agarose or porous glass beads, immobilizing the affinity ligand. The mobile...
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Coulomb Explosion Imaging as a Tool to Distinguish Between Stereoisomers
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Chiral Recognition for Chromatography and Membrane-Based Separations: Recent Developments and Future Prospects.

Yuan Zhao1, Xuecheng Zhu1, Wei Jiang1

  • 1Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University, 11 Fucheng Road, Beijing 100048, China.

Molecules (Basel, Switzerland)
|March 6, 2021
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Summary

Chiral separation is crucial for detecting harmful enantiomers in industries. This review details chromatography and membrane techniques for resolving chiral molecules, enhancing safety and application across various fields.

Keywords:
chiral separationchiral stationary phaseschromatographymembranerecognition

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Area of Science:

  • Analytical Chemistry
  • Separation Science
  • Biochemistry

Background:

  • Chiral molecules, like drugs and pesticides, pose risks due to differing biological effects of enantiomers.
  • Enantiomer-specific binding to receptors can lead to adverse health effects, such as endocrine disruption.
  • Accurate separation and detection of chiral substances are vital for industrial and environmental safety.

Purpose of the Study:

  • To review and detail chromatographic and membrane-based enantioseparation techniques.
  • To summarize the properties and applications of these chiral resolution methods.
  • To discuss recognition mechanisms and future prospects in chiral analysis.

Main Methods:

  • Focuses on chiral stationary phase (CSP) chromatography for enantiomer separation.
  • Details membrane-based enantiomer filtration techniques.
  • Reviews analytical enantioseparation mechanisms.

Main Results:

  • Summarizes unique properties of chromatographic and membrane methods for practical applications.
  • Highlights applications in chemistry, environment, biology, medicine, and food industries.
  • Analyzes recent advancements and future trends in enantioseparation.

Conclusions:

  • Chromatographic and membrane techniques are essential for chiral molecule resolution.
  • These methods offer significant potential for various industries requiring chiral analysis.
  • Further development in enantioseparation promises enhanced safety and product quality.