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Hedgehog Signaling Pathway

Hedgehog Signaling Pathway

The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...

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Related Experiment Video

Updated: Nov 15, 2025

Comparative Analysis of Human Growth Hormone in Serum Using SPRi, Nano-SPRi and ELISA Assays

Comparative Analysis of Human Growth Hormone in Serum Using SPRi, Nano-SPRi and ELISA Assays

Published on: January 7, 2016

The ElonginB/C-Cullin5-SOCS-Box-Complex Is a Potential Biomarker for Growth Hormone Disorders.

Wilhelm Gossing^1,2, Lars Radke¹, Henrik Biering^1,3

¹Division Molecular Biotechnology and Functional Genomics, Technical University of Applied Sciences, 15745 Wildau, Germany.

|March 6, 2021

Summary

New biomarkers, the elonginB/C-cullin5-SOCS-box-complex (ECS-complex) proteins, show promise for diagnosing acromegaly and growth hormone deficiency (GHD). Combining SOCS2, cullin-5, and Rbx-2 achieved perfect accuracy in distinguishing patients from controls.

Keywords:

SOCS2 acromegaly biomarker growth hormone deficiency liquid biopsies pituitary

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Area of Science:

Endocrinology
Biochemistry
Molecular Biology

Background:

Insulin-like growth factor 1 (IGF-1) is the standard biomarker for acromegaly and growth hormone deficiency (GHD).
Limitations of IGF-1 necessitate the search for novel, sensitive, and specific biomarkers.
The elonginB/C-cullin5-SOCS-box-complex (ECS-complex) is involved in regulating growth hormone (GH) receptor levels and cellular GH sensitivity.

Purpose of the Study:

To evaluate the diagnostic potential of individual ECS-complex proteins as biomarkers for acromegaly and GHD.
To assess the combined diagnostic performance of specific ECS-complex proteins in GH-related disorders.

Main Methods:

Measurement of individual ECS-complex proteins (SOCS2, cullin-5, Rbx-2) in whole blood samples using ELISA.
Comparison of protein levels in patients with acromegaly (n=32) and GHD (n=12) against healthy controls.
Application of hierarchical clustering to analyze combined protein expression data.

Main Results:

Suppressor of cytokine signaling 2 (SOCS2) demonstrated the highest individual diagnostic accuracy (0.93).
A combination of SOCS2, cullin-5, and ring-box protein 2 (Rbx-2) achieved 93% separation of patient samples from controls.
The combined three-protein panel provided perfect sensitivity and specificity for all patient groups, irrespective of IGF-1 levels or treatment status.

Conclusions:

ECS-complex proteins, particularly SOCS2, cullin-5, and Rbx-2, are potent novel biomarkers for diagnosing GH-related diseases.
These biomarkers offer superior diagnostic performance compared to IGF-1 in certain patient cohorts.
The ECS-complex plays a significant role in GH metabolism and its components are valuable for clinical diagnostics.