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FITC-Dextran Release from Cell-Embedded Fibrin Hydrogels.

Viki Raz Lepsky1, Sari Natan2, Oren Tchaicheeyan2

  • 1Department of Materials Science and Engineering, Tel-Aviv University, Tel-Aviv, 55 Chaim Levanon St., Ramat Aviv 69978, Israel.

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Adding cells to fibrin hydrogels slightly reduces biomolecule release, primarily due to gel degradation and diffusion obstruction. This finding aids in optimizing fibrin hydrogels for tissue engineering and drug delivery.

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Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Drug Delivery

Background:

  • Fibrin hydrogels are crucial in tissue engineering and drug delivery.
  • Previous research analyzed molecule release from fibrin, but cell impact remained unexplored.

Purpose of the Study:

  • To quantitatively assess the effect of embedded cells on biomolecule release from fibrin hydrogels.
  • To investigate the mechanisms modulating release profiles in cell-populated fibrin gels.

Main Methods:

  • Release of Fluorescein isothiocyanate (FITC)-dextran (250 kDa) from fibrin hydrogels with varying concentrations of fibroblasts or endothelial cells was monitored over 48 hours.
  • Analysis focused on the influence of cellular activities like gel degradation and physical obstruction.

Main Results:

  • Cellular incorporation into fibrin hydrogels resulted in a modest decrease in FITC-dextran release (7-15% for fibroblasts, 6-8% for endothelial cells) compared to acellular controls.
  • Gel degradation and physical obstruction to diffusion were identified as key modulators of release.
  • Cell-generated forces and matrix deformation did not significantly impact release profiles.

Conclusions:

  • Embedded cells cause minor alterations in biomolecule release from fibrin hydrogels.
  • Understanding these cellular effects, particularly gel degradation and diffusion hindrance, is vital for tailoring fibrin hydrogel performance in biomedical applications.
  • This research provides insights for predicting and controlling release kinetics in tissue engineering and drug delivery systems.