Related Experiment Video
Updated: Nov 15, 2025

Growing a Cystic Fibrosis-Relevant Polymicrobial Biofilm to Probe Community Phenotypes
Published on: April 19, 2024
[Development of a simple medium for the selective isolation of bacteria belonging to the Streptococcus anginosus
Elena M Berardinelli1, Horacio A Lopardo2
1Hospital General de Agudos Dr. Abel Zubizarreta, Ciudad Autónoma de Buenos Aires, Argentina; Hospital de Pediatría Prof. Dr. Juan P. Garrahan, Ciudad Autónoma de Buenos Aires, Argentina.
Abstract:
Bacteria of the Streptococcus anginosus group (SAG) have recently been recognized as contributors of bronchopulmonary disease in cystic fibrosis (CF). Routine detection and quantification are limited by current CF microbiology protocols. The development of the CHROMagar™ StrepB chromogenic medium modified by the addition of plasma, nalidixic acid, colistin and sulfadiazine to make it selective for SAG bacteria is described. This medium could be used as a diagnostic tool to detect SAG in respiratory samples from patients suffering from chronic lung disease.
Related Concept Videos
Techniques for Isolation of Pure Cultures
Antibiotic Selection
Microbial Growth Media
Overview Of Cell Separation And Isolation

