Development of a novel label-free impedimetric electrochemical sensor based on hydrogel/chitosan for the detection of ochratoxin A

Ochratoxin A (OTA) is one of the most abundant mycotoxins that contaminate various food products. Herein, we propose a novel label-free impedimetric electrochemical sensor consisting of chitosan/dipeptide nanofibrous hydrogel and immobilized DNA probes with OTA aptamer for the detection of OTA. The thin film of chitosan/dipeptide nanofibrous hydrogel was used as sensing interface and carrier for hybridization chain reaction (HCR) of OTA aptamer and DNA2 strand to form DNA concatemer. The concatemer was dissociated to single-stranded DNA (ssDNA) in the presence of target OTA, and the signal amplification was further implemented by introducing RecJ_f exonuclease, which could digest the single-stranded DNA resulting in OTA recycle. Electrochemical impedance spectroscopy (EIS) has been employed to characterize the properties of the fabricated sensor. A linear detection range of 0.1-100 ng mL^-1 was obtained for OTA with a low detection limit of 0.03 ng mL^-1. Furthermore, the developed sensor was demonstrated in white wine to detect OTA, indicating that the proposed impedimetric sensor has a promising potential application in the food industry.