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A Method for the Establishment of Human Lung Adenocarcinoma Patient-Derived Xenografts in Mice
Joanne Lundy1,2,3, Brendan J Jenkins1,2, Mohamed I Saad4,5
1Centre for Innate Immunity and Infectious Diseases, Hudson Institute of Medical Research, Clayton, VIC, Australia.
Abstract:
Patient-derived xenografts (PDXs) are created by implanting human tumor tissue or cells into immunodeficent mice, and enable the study of tumor biology, biomarkers and response to therapy in vivo. This chapter describes a method for lung adenocarcinoma (LAC) PDX generation using subcutaneous implantation of tumor tissue and cell suspensions and incorporating the humanization of PDX models by reconstitution with human immune cells.
