Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

953
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
953
CRISPR01:59

CRISPR

54.6K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
54.6K
CRISPR and crRNAs02:53

CRISPR and crRNAs

18.1K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
18.1K
Homologous Recombination02:31

Homologous Recombination

58.7K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
58.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Controlling GRF4-GIF1 expression for efficient, genotype-independent transformation across wheat cultivars.

The Plant journal : for cell and molecular biology·2026
Same author

Fine-tuning wheat development for the winter to spring transition.

Plant communications·2025
Same author

<i>DICER-LIKE 5</i> loss causes thermosensitive male sterility in durum wheat and reveals an AU-rich motif guiding 24-nt phasiRNA biogenesis.

Proceedings of the National Academy of Sciences of the United States of America·2025
Same author

Integrating genome editing with omics, artificial intelligence, and advanced farming technologies to increase crop productivity.

Plant communications·2025
Same author

Septoria tritici blotch resistance gene Stb15 encodes a lectin receptor-like kinase.

Nature plants·2025
Same author

An optimised CRISPR Cas9 and Cas12a mutagenesis toolkit for Barley and Wheat.

Plant methods·2024

Related Experiment Video

Updated: Nov 14, 2025

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits
09:43

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits

Published on: January 3, 2025

2.9K

CRISPR-Cas9 Based Genome Editing in Wheat.

Mark A Smedley1, Sadiye Hayta1, Martha Clarke1

  • 1John Innes Centre, Department of Crop Genetics, Norwich Research Park, Norwich, United Kingdom.

Current Protocols
|March 9, 2021
PubMed
Summary
This summary is machine-generated.

This study details CRISPR-Cas9 genome editing protocols for wheat, enabling precise gene knockout for crop improvement. The methods cover sgRNA design, construct assembly, and screening for successful edits in durum and bread wheat.

Keywords:
CRISPRCas9Golden Gate assemblygenome editingknockoutwheat

More Related Videos

CRISPR-Cas9-Mediated Genome Editing in the Filamentous Ascomycete Huntiella omanensis
07:25

CRISPR-Cas9-Mediated Genome Editing in the Filamentous Ascomycete Huntiella omanensis

Published on: June 9, 2020

9.8K
Embryo Microinjection and Knockout Mutant Identification of CRISPR/Cas9 Genome-Edited Helicoverpa Armigera H&#252;bner
06:37

Embryo Microinjection and Knockout Mutant Identification of CRISPR/Cas9 Genome-Edited Helicoverpa Armigera Hübner

Published on: July 1, 2021

4.8K

Related Experiment Videos

Last Updated: Nov 14, 2025

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits
09:43

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits

Published on: January 3, 2025

2.9K
CRISPR-Cas9-Mediated Genome Editing in the Filamentous Ascomycete Huntiella omanensis
07:25

CRISPR-Cas9-Mediated Genome Editing in the Filamentous Ascomycete Huntiella omanensis

Published on: June 9, 2020

9.8K
Embryo Microinjection and Knockout Mutant Identification of CRISPR/Cas9 Genome-Edited Helicoverpa Armigera H&#252;bner
06:37

Embryo Microinjection and Knockout Mutant Identification of CRISPR/Cas9 Genome-Edited Helicoverpa Armigera Hübner

Published on: July 1, 2021

4.8K

Area of Science:

  • Agricultural Science
  • Molecular Biology
  • Genetics

Background:

  • Genome editing tools like CRISPR-Cas9 are revolutionizing crop breeding and fundamental science.
  • Wheat, including durum and bread wheat, possesses large genomes with conserved homoeologs, making it amenable to targeted gene editing.
  • CRISPR-Cas9 technology enables precise, site-specific DNA modifications for gene knockout.

Purpose of the Study:

  • To provide experimentally derived protocols for implementing CRISPR-Cas9 genome editing in wheat.
  • To detail strategies for sgRNA design, Golden Gate construct assembly, and screening for genome edits.
  • To facilitate the application of CRISPR-Cas9 in wheat for agronomical and scientific research.

Main Methods:

  • Selection of single-guide RNA (sgRNA) target sequences for CRISPR-Cas9.
  • Construction of CRISPR-Cas9 editing vectors using Golden Gate (MoClo) assembly.
  • Screening methods for identifying successful CRISPR-Cas9-induced genome edits, including BigDye Terminator reactions.

Main Results:

  • Established protocols for CRISPR-Cas9 genome editing in wheat species (Triticum turgidum and Triticum aestivum).
  • Demonstrated successful application of sgRNA design, construct assembly, and screening for gene knockout.
  • Facilitated targeted modification of homoeologous genes within wheat's complex genome.

Conclusions:

  • The described protocols provide a robust strategy for CRISPR-Cas9 genome editing in wheat.
  • This approach supports the advancement of crop improvement and fundamental research in wheat genetics.
  • CRISPR-Cas9 technology offers significant potential for accelerating genetic advancements in major wheat varieties.