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Related Concept Videos

Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

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Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
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Related Experiment Video

Updated: Nov 14, 2025

Improved UPLC-UV Method for the Quantification of Vitamin C in Lettuce Varieties Lactuca sativa L. and Crop Wild Relatives Lactuca spp.
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Novel LC-MS-TOF method to detect and quantify ascorbic and uric acid simultaneously in different biological matrices.

Eva Borras1, Leah Schrumpf2, Noelle Stephens2

  • 1Mechanical and Aerospace Engineering, University of California, Davis, Davis, CA, USA.

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
|March 10, 2021
PubMed
Summary

A new liquid chromatography-mass spectrometry method accurately quantifies ascorbic acid (AA) and uric acid (UA) in biological fluids. This technique is validated for plasma, exhaled breath condensate, and nasal lavage, enabling precise antioxidant analysis.

Keywords:
Ascorbic acidBiological matricesExhaled breath condensate (EBC)LC-MS-TOFNasal lavagePlasmaQuantificationUric acidValidation

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Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry UPLC-MS
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Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Biomedical Science

Background:

  • Ascorbic acid (AA) and uric acid (UA) are key antioxidants in biological fluids.
  • Accurate quantification of these antioxidants is crucial for understanding biological processes and disease states.

Purpose of the Study:

  • To develop and validate a novel liquid chromatography-mass spectrometry with time-of-flight (LC-MS-TOF) method for simultaneous quantification of AA and UA.
  • To apply the method to various biological matrices including plasma, exhaled breath condensate (EBC), and nasal lavage (NL).

Main Methods:

  • Simultaneous quantification of AA and UA using LC-MS-TOF.
  • Protein precipitation using MPA, antioxidant solution, and acetonitrile.
  • Reverse-phase C18 column chromatography with a water/acetonitrile gradient elution.
  • Detection and quantification in negative electrospray ionization (ESI) mode with isotope-labeled internal standards.

Main Results:

  • The method demonstrated good selectivity, linearity, accuracy, precision, recovery, and matrix effect for AA and UA.
  • Validated linear ranges were established for plasma (1-25 µg/mL for AA, 1-40 µg/mL for UA) and EBC/NL (0.05-5 µg/mL for AA, 0.05-7.5 µg/mL for UA).
  • The method was successfully applied to real biological samples, revealing higher antioxidant concentrations in plasma compared to EBC and NL.

Conclusions:

  • A novel, validated LC-MS-TOF method allows for the simultaneous quantification of ascorbic acid and uric acid in biological samples.
  • The method is suitable for small sample volumes (<250 µL) and various biological matrices.
  • This technique provides a reliable tool for antioxidant research and clinical diagnostics.