Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Imaging Biological Samples with Optical Microscopy

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Corrigendum for the article Clin Nephrol. 2025; 104: 318-327.

Clinical nephrology·2026

Same author

Design and theoretical analysis of a field-widened cross-dispersed spatial heterodyne spectrometer based on multi-gratings and a postposed transmission grating.

Optics express·2026

Same author

Detection of microplastics via a frequency-shifted excitation confocal micro-differential, spatial heterodyne, Raman spectrometer with echelle-mirror structure.

Optics express·2026

Same author

Phase I Studies of HLX26, A Novel Anti-LAG-3 Antibody, Monotherapy or Combination Therapy in Patients with Advanced Solid Tumors.

BioDrugs : clinical immunotherapeutics, biopharmaceuticals and gene therapy·2026

Same author

A predictive model for olfactory dysfunction in patients undergoing maintenance hemodialysis.

Clinical nephrology·2025

Same author

Raman measurements using an acousto-optic tunable filter spatial heterodyne Raman spectrometer with an echelle-mirror structure.

Optics express·2025

Same journal

Multifunctional reconfigurable terahertz metasurface based on vanadium dioxide phase transition: achieving broadband absorption and efficient polarization conversion.

Applied optics·2026

Same journal

High-Q-factor electromagnetically induced transparency utilizing quasi-bound states in the continuum in an all-dielectric terahertz metasurface.

Applied optics·2026

Same journal

Automated stitching interferometry for high-precision metrology of X-ray mirrors.

Applied optics·2026

Same journal

Experimental demonstration of an approach to designing a metal-dielectric DBR resonant cavity structure.

Applied optics·2026

Same journal

High-precision wavefront reconstruction from a single-shot interferogram using a physics-driven hybrid feature calibration network.

Applied optics·2026

Same journal

Ultra-high-Q Fano resonance based on coupled topological corner states in Kagome photonic crystals.

Applied optics·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Nov 14, 2025

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

Published on: July 5, 2016

Microlens array snapshot hyperspectral microscopy system for the biomedical domain.

Changben Yu, Jin Yang, Nan Song

|March 10, 2021

Summary

This summary is machine-generated.

This study introduces a novel snapshot hyperspectral microscope using a microlens array for rapid 3D data cube acquisition in biomedical imaging. The system achieves high spatial and spectral resolution for real-time biological analysis.

More Related Videos

Excitation-Scanning Hyperspectral Imaging Microscopy to Efficiently Discriminate Fluorescence Signals

Excitation-Scanning Hyperspectral Imaging Microscopy to Efficiently Discriminate Fluorescence Signals

Published on: August 22, 2019

Lensless On-chip Imaging of Cells Provides a New Tool for High-throughput Cell-Biology and Medical Diagnostics

Lensless On-chip Imaging of Cells Provides a New Tool for High-throughput Cell-Biology and Medical Diagnostics

Published on: December 14, 2009

Related Experiment Videos

Last Updated: Nov 14, 2025

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

Published on: July 5, 2016

Excitation-Scanning Hyperspectral Imaging Microscopy to Efficiently Discriminate Fluorescence Signals

Excitation-Scanning Hyperspectral Imaging Microscopy to Efficiently Discriminate Fluorescence Signals

Published on: August 22, 2019

Lensless On-chip Imaging of Cells Provides a New Tool for High-throughput Cell-Biology and Medical Diagnostics

Lensless On-chip Imaging of Cells Provides a New Tool for High-throughput Cell-Biology and Medical Diagnostics

Published on: December 14, 2009

Area of Science:

Biomedical Optics
Microscopy
Spectroscopy

Background:

Hyperspectral imaging is crucial for analyzing biological samples.
Existing methods often face limitations in speed and spatial resolution.

Purpose of the Study:

To develop a microlens array-type snapshot hyperspectral microscope system.
To enable rapid spatial-spectral sampling for biomedical applications.

Main Methods:

Utilized a shared optical path design for simultaneous direct imaging and hyperspectral data collection.
Employed a microlens array coupled with a prism-grating hyperspectral imager for spectral dispersion.
Achieved rapid acquisition of 3D data cubes (x×y×λ) at detector frame rates.

Main Results:

Demonstrated a spatial resolution of 2.5 µm.
Achieved 180-channel spectral sampling across a 100 nm range (400-800 nm) with ~0.56 nm spectral resolution.
Successfully acquired 3D spectral data cubes (28×14×180) rapidly.

Conclusions:

The microlens array-type snapshot hyperspectral microscope system is suitable for rapid 3D data acquisition.
The system shows potential for real-time biological spectral imaging applications.