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Published on: October 22, 2018
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Elevated CO2 differentially regulates root nitrate transporter kinetics in a genotype and nitrate dose-dependent
Sandeep B Adavi1, Lekshmy Sathee1
1Division of Plant Physiology, Indian Agricultural Research Institute, New Delhi, 110012, India.
Summary
Elevated CO2 (EC) reduces wheat nitrogen and protein by increasing nitric oxide (NO), which suppresses nitrate uptake. This effect is genotype and nitrate-dependent, impacting low-affinity nitrate transporters (LATS).
Area of Science:
- Plant Physiology
- Agricultural Science
- Environmental Science
Background:
- Elevated CO2 (EC) often decreases nitrogen (N) and protein content in wheat.
- Previous research linked EC to increased nitric oxide (NO), repressing nitrate reductase (NR) and high-affinity nitrate transporters (HATS) under high N.
- EC with high N also caused reactive oxygen and nitrogen species (ROS/RNS) accumulation, leading to faster senescence and reduced N metabolites.
Purpose of the Study:
- Investigate the short-term effects of EC on nitrate uptake kinetics in wheat.
- Determine the impact of EC on constitutive and inducible components of high-affinity (HATS) and low-affinity nitrate uptake systems (LATS).
- Delineate these effects in two wheat genotypes with differing nitrate uptake and assimilation capacities.
Main Methods:
- Studied nitrate uptake kinetics under short-term elevated CO2 exposure.
- Assessed the impact of EC on HATS and LATS components in two wheat genotypes.
- Measured nitrate dose-response of NR, NO abundance, and exogenous NO supply effects.
Main Results:
- EC suppressed nitrate dose-response of NR in both leaf and root tissues.
- Plants under EC showed reduced LATS kinetic components.
- A high nitrate assimilation genotype maintained higher nitrate uptake under EC, though at a reduced rate compared to ambient CO2.
- EC-exposed leaves had higher NO abundance, increasing with nitrate supply.
- Exogenous NO supply significantly reduced nitrate uptake in EC-grown plants.
Conclusions:
- Elevated CO2 downregulates low-affinity nitrate transporter (LATS) kinetics in wheat.
- This downregulation is mediated by EC-induced nitric oxide (NO) production.
- The effect is dependent on wheat genotype and nitrate availability.
Keywords:
Elevated CO(2)High affinity nitrate transport systemK(m)Low affinity nitrate transport systemNitrate uptake kineticsV(max)WheatMore Related Videos
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