Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A genome-scale CRISPRi perturbation atlas of human induced pluripotent stem cells.

Nature biotechnology·2026
Same author

Receptor-Guided AAV Tropism Engineering via MATCH.

ACS synthetic biology·2026
Same author

Validation of DoriVac (DNA Origami Vaccine) Efficacy in a Metastatic Melanoma Model.

ACS applied materials & interfaces·2026
Same author

Receptor-guided AAV Tropism Engineering via MATCH.

bioRxiv : the preprint server for biology·2026
Same author

DNA origami vaccine nanoparticles improve humoral and cellular immune responses to infectious diseases.

Nature biomedical engineering·2026
Same author

Human Dorsal Root Ganglia Neuronal Cell Line to Study Nociceptive Signaling: A New Pipeline for Pain Therapy.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2026

Related Experiment Video

Updated: Nov 14, 2025

Lineage Tracing and Clonal Analysis in Developing Cerebral Cortex Using Mosaic Analysis with Double Markers MADM
09:25

Lineage Tracing and Clonal Analysis in Developing Cerebral Cortex Using Mosaic Analysis with Double Markers MADM

Published on: May 8, 2020

11.0K

Lineage barcoding in mice with homing CRISPR.

Kathleen Leeper1,2, Kian Kalhor3, Andyna Vernet4

  • 1Department of Biomedical Engineering, Johns Hopkins University School of Medicine, Baltimore, MD, USA.

Nature Protocols
|March 11, 2021
PubMed
Summary
This summary is machine-generated.

We developed a novel developmental barcoding system using MARC1 mouse lines to trace cell lineages. This method employs synthetically induced mutations for comprehensive lineage tracing in vivo.

More Related Videos

Efficient Generation of hiPSC Neural Lineage Specific Knockin Reporters Using the CRISPR/Cas9 and Cas9 Double Nickase System
14:46

Efficient Generation of hiPSC Neural Lineage Specific Knockin Reporters Using the CRISPR/Cas9 and Cas9 Double Nickase System

Published on: May 28, 2015

11.4K
CRISPR/Cas9-mediated Targeted Integration In Vivo Using a Homology-mediated End Joining-based Strategy
08:22

CRISPR/Cas9-mediated Targeted Integration In Vivo Using a Homology-mediated End Joining-based Strategy

Published on: March 12, 2018

15.3K

Related Experiment Videos

Last Updated: Nov 14, 2025

Lineage Tracing and Clonal Analysis in Developing Cerebral Cortex Using Mosaic Analysis with Double Markers MADM
09:25

Lineage Tracing and Clonal Analysis in Developing Cerebral Cortex Using Mosaic Analysis with Double Markers MADM

Published on: May 8, 2020

11.0K
Efficient Generation of hiPSC Neural Lineage Specific Knockin Reporters Using the CRISPR/Cas9 and Cas9 Double Nickase System
14:46

Efficient Generation of hiPSC Neural Lineage Specific Knockin Reporters Using the CRISPR/Cas9 and Cas9 Double Nickase System

Published on: May 28, 2015

11.4K
CRISPR/Cas9-mediated Targeted Integration In Vivo Using a Homology-mediated End Joining-based Strategy
08:22

CRISPR/Cas9-mediated Targeted Integration In Vivo Using a Homology-mediated End Joining-based Strategy

Published on: March 12, 2018

15.3K

Area of Science:

  • Developmental Biology
  • Genomics
  • Molecular Biology

Background:

  • Traditional in vivo cell lineage tracing methods are often complex and provide limited temporal or trajectory data.
  • Developmental barcoding offers a powerful alternative for reconstructing cellular histories.

Purpose of the Study:

  • To detail the MARC1 (Mutant Allele Reporting via CRISPR) mouse lines for developmental barcoding.
  • To provide experimental strategies and protocols for utilizing MARC1 mice in lineage analysis.

Main Methods:

  • Utilizing MARC1 mouse lines with multiple homing guide RNAs to generate diverse, synthetically induced mutations.
  • Developing a protocol for barcode retrieval, sequencing, and subsequent data analysis.
  • Employing CRISPR-Cas9 technology for targeted genomic alterations.

Main Results:

  • Two specific MARC1 mouse lines are now available from a public repository.
  • The described protocol enables the generation of barcodes from synthetically induced mutations for lineage tracing.
  • The system allows for exponential diversity of barcodes, facilitating detailed lineage reconstruction.

Conclusions:

  • MARC1 mouse lines provide an advanced tool for in vivo lineage tracing.
  • This barcoding system overcomes limitations of traditional methods, offering a more comprehensive view of cellular development.
  • The detailed protocols facilitate the adoption of this technique in research settings.