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Updated: Nov 14, 2025

Detection and Isolation of Circulating Melanoma Cells using Photoacoustic Flowmetry

Detection and Isolation of Circulating Melanoma Cells using Photoacoustic Flowmetry

Published on: November 25, 2011

Transcript-Based Detection of Circulating Melanoma Cells.

Michael Morici¹, Weitao Lin¹, Elin S Gray²

¹School of Medical and Health Sciences, Edith Cowan University, Joondalup, WA, Australia.

Methods in Molecular Biology (Clifton, N.J.)

|March 11, 2021

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Detecting circulating tumor cells (CTCs) in melanoma patients is crucial for early metastasis detection and prognostication. This study presents a new transcript analysis method for improved melanoma CTC detection in enriched blood samples.

Area of Science:

Oncology
Molecular Biology
Cancer Diagnostics

Background:

Circulating tumor cells (CTCs) are shed from primary tumors and can initiate metastasis.
Detecting CTCs aids in early diagnosis and prognosis of metastatic disease.
Accurate detection of melanoma CTCs requires sensitive and reliable methodologies.

Purpose of the Study:

To develop and describe a novel method for analyzing circulating tumor cells (CTCs) in melanoma.
To enhance the detection sensitivity and reliability of melanoma CTCs.
To explore the biomarker potential of melanoma CTCs through transcript analysis.

Main Methods:

Enrichment of CTCs from peripheral blood samples.
Analysis of CTCs using melanoma-specific transcript detection.

Keywords:

Circulating tumor cell (CTC)Droplet digital PCR (ddPCR )Melanoma Real-time PCR Transcripts

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Last Updated: Nov 14, 2025

Detection and Isolation of Circulating Melanoma Cells using Photoacoustic Flowmetry

Detection and Isolation of Circulating Melanoma Cells using Photoacoustic Flowmetry

Published on: November 25, 2011

Patient Derived Cell Culture and Isolation of CD133+ Putative Cancer Stem Cells from Melanoma

Patient Derived Cell Culture and Isolation of CD133+ Putative Cancer Stem Cells from Melanoma

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A Robust Discovery Platform for the Identification of Novel Mediators of Melanoma Metastasis

Published on: March 8, 2022

Quantification of specific transcripts to identify and measure CTC presence.

Main Results:

The described method enables the analysis of CTCs in enriched melanoma patient samples.
Melanoma-specific transcript analysis proves effective for CTC detection.
The findings support the utility of transcript analysis for CTC assessment.

Conclusions:

The developed method offers a sensitive approach for melanoma CTC detection.
Transcript analysis of CTCs provides valuable insights for disease prognostication.
This technique advances the potential for early metastatic dissemination detection in melanoma.