The Histo-CLEM Workflow for tissues of model organisms

Area of Science:

• Biological imaging
• Microscopy techniques
• Tissue analysis

Background:

• Bridging macrostructure and nanostructure in tissues presents significant technical challenges.
• Existing methods often lack the flexibility to analyze diverse biological samples across various length scales.

Purpose of the Study:

• To develop a versatile workflow for correlative light and electron microscopy (CLEM) applicable to diverse tissues and model organisms.
• To provide a detailed protocol for the Histo-CLEM workflow, enabling researchers to adapt it for specific biological questions and tissue types.

Main Methods:

• The Histo-CLEM Workflow integrates histology, light microscopy, and electron microscopy.
• The workflow is designed to be flexible, allowing adaptation for different tissue types and research objectives.
• Detailed procedural steps are provided for method implementation.

Main Results:

• A flexible Histo-CLEM workflow was successfully developed and validated.
• The workflow enables analysis of tissues across multiple length scales, from macrostructure to nanostructure.
• Demonstrated application in the preparation and visualization of mouse nerve fibers.

Conclusions:

• The Histo-CLEM Workflow offers a robust solution for challenging multi-scale tissue analysis.
• This integrated microscopy approach enhances the study of tissue architecture and cellular ultrastructure.
• The method's adaptability makes it valuable for a wide range of biological research applications.