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Complete Genome Sequence of a Type III Ovine Strain of Mycobacterium avium subsp. paratuberculosis

John P Bannantine1, Darrell O Bayles2, Franck Biet3

  • 1USDA-Agricultural Research Service, National Animal Disease Center, Ames, Iowa, USA john.bannantine@usda.gov.

Microbiology Resource Announcements
|March 12, 2021
PubMed

Insights

The complete genome of Mycobacterium avium subsp. paratuberculosis, a sheep pathogen, was sequenced. This analysis revealed its chromosome size and 19 copies of the IS900 element, crucial for identification.

Area of Science:

  • Genomics
  • Microbiology
  • Veterinary Science

Background:

  • Mycobacterium avium subsp. paratuberculosis (MAP) is an important animal pathogen.
  • Accurate identification of MAP is crucial for disease control in livestock.

Purpose of the Study:

  • To determine the complete genome sequence of a type III strain of MAP.
  • To characterize the genomic features of this important sheep pathogen.

Main Methods:

  • Whole-genome sequencing of the MAP type III strain.
  • Bioinformatic analysis of the resulting genome sequence.

Main Results:

  • The complete genome size of the MAP strain is 4,895,755 base pairs.
  • No plasmid DNA was detected in this strain.
  • The chromosome contains 19 copies of the IS900 insertion sequence element.

Conclusions:

  • The genome sequence provides a comprehensive resource for studying MAP.
  • The presence and number of IS900 copies are confirmed, reinforcing its utility in MAP identification.

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