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Related Concept Videos

RNA-seq03:21

RNA-seq

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
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The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
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Multiplexed Single Cell mRNA Sequencing Analysis of Mouse Embryonic Cells
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Decoding Neuronal Diversification by Multiplexed Single-cell RNA-Seq.

Joachim Luginbühl1, Tsukasa Kouno1, Rei Nakano2

  • 1RIKEN Center for Integrative Medical Sciences, Yokohama, Kanagawa 230-0045, Japan; RIKEN Center for Life Science Technologies, Division of Genomic Technologies, Yokohama, Kanagawa 230-0045, Japan.

Stem Cell Reports
|March 12, 2021
PubMed
Summary
This summary is machine-generated.

Researchers reprogrammed human fibroblasts into diverse neuron subtypes using 20 transcription factors. Single-cell RNA sequencing revealed key regulators of neuronal lineage specification and diversification, offering insights into brain development.

Keywords:
direct cell reprogrammingneuronal reprogrammingsingle-cell multiplexing genomics

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Area of Science:

  • Neuroscience
  • Genetics
  • Molecular Biology

Background:

  • Cellular reprogramming is crucial for understanding cell-type specification.
  • The regulatory logic governing neuronal diversification remains largely unknown.
  • Single-cell RNA sequencing (scRNA-seq) offers high-resolution insights into dynamic molecular changes.

Purpose of the Study:

  • To investigate the regulatory logic of neuronal diversification using multiplex transcription factor expression.
  • To identify key determinants of neuronal lineage specification.
  • To explore reprogramming processes correlating with brain development.

Main Methods:

  • Ectopic expression of 20 pro-neuronal transcription factors in human dermal fibroblasts.
  • Multiplex single-cell RNA sequencing (scRNA-seq) for high-resolution analysis.
  • Gene mapping to identify regulatory transcription factors.

Main Results:

  • Demonstrated widespread diversification of neurons with distinct morphologies and marker expressions.
  • Identified diverse neuronal subtypes, including those resembling developing brain cells.
  • Uncovered key transcription factors regulating glutamatergic and cholinergic neuron specification.

Conclusions:

  • Multiplex scRNA-seq is a powerful, scalable method for studying cellular specification.
  • Elucidated critical regulatory logic governing neuronal lineage diversification.
  • Provided insights into mechanisms underlying neuronal subtype generation.