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Synthesis of Keratin-based Nanofiber for Biomedical Engineering
Published on: February 7, 2016
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Newer guar gum ester/chicken feather keratin interact films for tissue engineering
Aatrayee Das1, Ankita Das2, Aalok Basu3
1Division of Pharmaceutical and Fine Chemical Technology, Department of Chemical Technology, University of Calcutta, 92, A.P.C. Road, Kolkata 700009, West Bengal, India.
International Journal of Biological Macromolecules
|March 12, 2021
Summary
Researchers synthesized a novel guar gum indole acetate (GGIA) biopolymer and developed protein-polysaccharide film scaffolds. These biocompatible GGIA-keratin films show promise for skin tissue engineering due to their porosity and antimicrobial properties.
Area of Science:
- Biomaterials Science
- Polymer Chemistry
- Tissue Engineering
Background:
- Developing advanced biomaterials is crucial for effective tissue engineering.
- Protein-polysaccharide interactions offer unique properties for scaffold design.
- Sustainable sourcing of biomaterials from waste is an important research area.
Purpose of the Study:
- To synthesize a novel guar gum indole acetate ester (GGIA).
- To design and characterize protein-polysaccharide film scaffolds for tissue engineering.
- To evaluate the biocompatibility and antimicrobial activity of the developed films.
Main Methods:
- Guar gum was modified to synthesize GGIA using aprotic solvent activated hofmeister ions.
- GGIA was characterized using FT-IR, 13C NMR, XRD, and TGA.
- GGIA was cross-linked with hydrolyzed keratin to form film scaffolds, which were then tested for porosity, tensile strength, biocompatibility, and antimicrobial activity.
Main Results:
- A novel biopolymer, GGIA, with a high Degree of Substitution (DS = 0.61) was successfully synthesized and characterized.
- GGIA-keratin films exhibited favorable physicochemical properties, high porosity (63%), and good tensile strength (6.4 MPa).
- The bio-based films demonstrated non-cytotoxicity, excellent biocompatibility with human dermal fibroblasts, and significant antimicrobial activity against E. coli and S. aureus.
Conclusions:
- GGIA-keratin film scaffolds are promising, fully bio-based materials for skin tissue engineering.
- The developed scaffolds possess desirable mechanical, biological, and antimicrobial properties.
- This study highlights the potential of utilizing waste-derived materials for advanced biomedical applications.
