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Pocket MUSE: an affordable, versatile and high-performance fluorescence microscope using a smartphone.

Yehe Liu1, Andrew M Rollins1, Richard M Levenson2

  • 1Department of Biomedical Engineering, Case Western Reserve University, Cleveland, OH, USA.

Communications Biology
|March 13, 2021
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Summary

Pocket MUSE integrates Microscopy with Ultraviolet Surface Excitation (MUSE) into a smartphone microscope. This low-cost, portable device offers high-quality fluorescence imaging for diverse applications, from diagnostics to education.

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Area of Science:

  • Optics and Photonics
  • Biomedical Imaging
  • Consumer Electronics Integration

Background:

  • Smartphone microscopy offers potential for portable imaging.
  • Existing smartphone microscopes have limitations in resolution and fluorescence capabilities.
  • Microscopy with Ultraviolet Surface Excitation (MUSE) is a powerful fluorescence technique.

Purpose of the Study:

  • To demonstrate the first practical implementation of MUSE in a smartphone microscope.
  • To develop a low-cost, compact, and user-friendly fluorescence microscopy system.
  • To explore the versatility of the system for various point-of-care and educational applications.

Main Methods:

  • Fabrication of a compact optical module using readily available consumer electronics parts.
  • Integration of the MUSE technique with a smartphone's camera.
  • Development of simple, portable sample preparation strategies.

Main Results:

  • Successful creation of Pocket MUSE, a smartphone-based fluorescence microscope.
  • Achieved submicron resolution with a 10x equivalent field of view.
  • Demonstrated high-quality, multichannel fluorescence imaging capabilities.

Conclusions:

  • Pocket MUSE provides an effective and affordable platform for advanced fluorescence microscopy.
  • The system is suitable for a wide range of applications including diagnostics, health monitoring, and education.
  • This technology democratizes access to powerful imaging tools.