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DNA Isolation

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...

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Related Experiment Video

Updated: Nov 13, 2025

On-site DNA Detection of Trypanosomatid Parasites and Nosema ceranae Through Alkaline Lysis Coupled to RPA/CRISPR/Cas12a System

On-site DNA Detection of Trypanosomatid Parasites and Nosema ceranae Through Alkaline Lysis Coupled to RPA/CRISPR/Cas12a System

Published on: July 18, 2025

Rapid parasite detection utilizing a DNA dipstick.

Oyime Poise Aula¹, Donald Peter McManus², Michael Glenn Mason³

¹QIMR Berghofer Medical Research Institute, Molecular Parasitology Laboratory, Brisbane, Australia; University of Queensland, Faculty of Medicine, Herston, 4006, QLD, Australia.

Experimental Parasitology

|March 13, 2021

Summary

This study optimized DNA dipstick technology for rapid Schistosoma japonicum detection. The fast, simple method allows for point-of-care diagnosis of parasitic worm infections, aiding disease control.

Keywords:

DNA dipstick LAMP Point-of-care testing Schistosoma japonicum qPCR

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Area of Science:

Molecular Diagnostics
Parasitology
Biotechnology

Background:

Molecular diagnostics are crucial for disease detection but often require laboratory settings.
Accurate diagnosis of parasitic worm infections, like Schistosoma japonicum, is vital for control programs and outbreak management.

Purpose of the Study:

To optimize DNA dipstick technology for rapid nucleic acid purification.
To enable point-of-care detection of Schistosoma japonicum.

Main Methods:

Optimization of DNA dipstick technology.
Purification of Schistosoma japonicum DNA from various life stages (adult worms, eggs, infected snails).

Main Results:

Successfully purified Schistosoma japonicum DNA in under 60 seconds.
Demonstrated successful DNA detection from adult worms, eggs, and infected snails.

Conclusions:

The optimized DNA dipstick technology provides a rapid and simple method for Schistosoma japonicum detection.
This advancement facilitates point-of-care diagnostics for parasitic worm infections.