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Updated: Nov 13, 2025
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Detection of Culturable Bacteria from Tomato Seeds on Media Semi-selective for the Canker Pathogen
Nobutaka Someya1, Masaharu Kubota1, Tomohiro Morohoshi2
1Institute of Vegetable and Floriculture Science, National Agriculture and Food Research Organization (NARO).
Abstract:
Culturable bacteria were isolated from tomato seeds using media selective for the canker pathogen Clavibacter michiganensis subsp. michiganensis. Clustering analysis (>99% identity) revealed the presence of 16 operational taxonomic units (OTUs) among isolates detected on semi-selective media. Three OTUs belonged to the phylum Actinobacteria, including those of Micrococcus and Dermacoccus, and 13 OTUs belonged to the phylum Firmicutes, including Bacillus and related genera. These Gram-positive endophytic bacteria have the potential to provide false-positive results in seed health tests using media considered semi-selective for the cancer pathogen.
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