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Targeting miR-148b-5p Inhibits Immunity Microenvironment and Gastric Cancer Progression

Yuyu Zhang1, Wei Huo1, Lidi Sun1

  • 1Department of Radiation Oncology, The First Hospital of Jilin University, Changchun, China.

Frontiers in Immunology
|March 15, 2021
PubMed
Abstract

Insights

MicroRNA-148b-5p (miR-148b-5p) is downregulated in gastric cancer (GC), inhibiting tumor progression and affecting drug sensitivity. Restoring miR-148b-5p levels shows potential for GC treatment.

Area of Science:

  • Oncology
  • Molecular Biology
  • Immunology

Background:

  • MicroRNAs (miRNAs) play critical roles in tumor development.
  • Limited research exists on miRNA involvement in gastric cancer (GC) progression.

Purpose of the Study:

  • Investigate the role of miR-148b-5p in gastric cancer (GC) development.
  • Determine if miR-148b-5p can be a therapeutic target for GC.

Main Methods:

  • Analyzed GC cell lines and patient samples to assess miR-148b-5p levels.
  • Performed in vitro and in vivo experiments to evaluate the effects of miR-148b-5p overexpression on GC.
  • Identified direct targets of miR-148b-5p using mechanistic studies.

Main Results:

  • miR-148b-5p was significantly downregulated in GC.
  • Overexpression of miR-148b-5p inhibited GC cell proliferation and invasion.
  • miR-148b-5p reprogrammed GC metabolism and modulated the immune microenvironment.
  • ATPIF1 was identified as a direct target of miR-148b-5p.
  • Low miR-148b-5p levels correlated with poor GC patient prognosis and altered sensitivity to anti-cancer drugs.

Conclusions:

  • miR-148b-5p acts as a tumor suppressor in GC.
  • Targeting miR-148b-5p may offer a novel therapeutic strategy for GC by modulating the immune microenvironment and inhibiting tumor progression.
Keywords:
ATPIF1gastric cancerimmune microenvironmentmetabolic reprogrammingmiR-148b-5p

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