Related Experiment Video
Updated: Nov 13, 2025
Simultaneous Study of the Recruitment of Monocyte Subpopulations Under Flow In Vitro
Published on: November 26, 2018
Monocyte Subset Recruitment Marker Profile Is Inversely Associated With Blood ApoA1 Levels
Vyoma K Patel1,2, Helen Williams1,2, Stephen C H Li3,4
1Vascular Biology Research Centre, Department of Surgery, Westmead Hospital, Westmead, NSW, Australia.
Insights
Dyslipidemia, particularly low Apolipoprotein A1 (ApoA1) levels, increases monocyte extravasation potential. This suggests dyslipidemia may enhance monocyte recruitment in cardiovascular disease development.
Area of Science:
- Cardiovascular Biology
- Immunology
- Lipid Metabolism
Background:
- Dyslipidemia is a key driver of atherosclerosis, characterized by plaque development.
- Monocyte influx into the vessel wall is crucial for plaque progression.
- The link between dyslipidemia and monocyte extravasation potential remains unclear.
Purpose of the Study:
- To investigate if dyslipidemia is associated with an increased potential for monocyte extravasation.
- To examine recruitment marker expression on monocyte subsets in relation to lipid profiles.
Main Methods:
- Flow cytometry was used to analyze monocyte subsets from healthy individuals with varying lipid profiles.
- Expression of recruitment markers was compared across monocyte subsets and participants.
- Correlations between marker expression and lipid levels, specifically Apolipoprotein A1 (ApoA1), were assessed.
Main Results:
- Monocyte subsets exhibited distinct recruitment marker expression patterns.
- Significant inter-participant variability in marker expression was observed, overshadowing subset differences.
- Higher expression of multiple recruitment markers correlated with lipid profiles, notably an inverse correlation with ApoA1 levels.
Conclusions:
- Dyslipidemia, especially low ApoA1, is linked to an elevated capacity for all monocyte subsets to extravasate.
- This extravasation involves a broader range of recruitment markers than previously recognized.
- Findings highlight a potential mechanism linking dyslipidemia to enhanced monocyte recruitment in cardiovascular disease.
Abstract:
Dyslipidemia promotes development of the atherosclerotic plaques that characterise cardiovascular disease. Plaque progression requires the influx of monocytes into the vessel wall, but whether dyslipidemia is associated with an increased potential of monocytes to extravasate is largely unknown. Here (using flow cytometry) we examined recruitment marker expression on monocytes from generally healthy individuals who differed in lipid profile. Comparisons were made between monocyte subsets, participants and relative to participants' lipid levels. Monocyte subsets differed significantly in their expression of recruitment markers, with highest expression being on either the classical or non-classical subsets. However, these inter-subset differences were largely overshadowed by considerable inter-participant differences with some participants having higher levels of recruitment markers on all three monocyte subsets. Furthermore, when the expression of one recruitment marker was high, so too was that of most of the other markers, with substantial correlations evident between the markers. The inter-participant differences were explained by lipid levels. Most notably, there was a significant inverse correlation for most markers with ApoA1 levels. Our results indicate that dyslipidemia, in particular low levels of ApoA1, is associated with an increased potential of all monocyte subsets to extravasate, and to do so using a wider repertoire of recruitment markers than currently appreciated.
More Related Videos
09:41Quantification of Monocyte Transmigration and Foam Cell Formation from Individuals with Chronic Inflammatory Conditions
Published on: October 17, 2017
09:57Quantification of Monocyte Chemotactic Activity In Vivo and Characterization of Blood Monocyte Derived Macrophages
Published on: August 12, 2019
