Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Types of Biopharmaceutical Studies: Controlled and Non-Controlled Approaches01:23

Types of Biopharmaceutical Studies: Controlled and Non-Controlled Approaches

249
Biopharmaceutical studies constitute a vital field aiming to enhance drug delivery methods and refine therapeutic approaches, drawing upon diverse interdisciplinary knowledge. In research methodologies, the choice between controlled and non-controlled studies significantly influences the study's reliability and accuracy.
Non-controlled studies, commonly employed for initial exploration, lack a control group, rendering them susceptible to biases and external influences. In contrast,...
249
Measurement of Bioavailability: Pharmacodynamic Methods01:20

Measurement of Bioavailability: Pharmacodynamic Methods

71
Pharmacodynamic methods provide insights into a drug's effects on physiological processes over time and play a crucial role in understanding bioavailability and therapeutic efficacy. These methods can be broadly classified into acute pharmacological and therapeutic response approaches, each with distinct mechanisms and applications.The acute pharmacological response method directly correlates a drug's physiological effects, such as ECG or pupil diameter changes, to its time course in the body.
71

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Authenticity, absence, and pedagogy on a historical injustice bus tour.

Memory studies·2025
Same author

The chordoid glioma <i>PRKCA</i> D463H mutation is a kinase inactive, gain-of-function allele that induces early-onset chondrosarcoma in mice.

Science signaling·2025
Same author

Penetrant PKCβ mutation in ATLL displays a mixed gain-of-function.

The Biochemical journal·2025
Same author

Historical thinking: Trends, critiques, and future directions.

Current opinion in psychology·2025
Same author

Electrochemical microfluidic biosensor for the detection of CD4<sup>+</sup> T cells.

Microsystems & nanoengineering·2025
Same author

Capture, mutual inhibition and release mechanism for aPKC-Par6 and its multisite polarity substrate Lgl.

Nature structural & molecular biology·2025

Related Experiment Video

Updated: Nov 12, 2025

Preparation of Peripheral Blood Mononuclear Cell Pellets and Plasma from a Single Blood Draw at Clinical Trial Sites for Biomarker Analysis
07:40

Preparation of Peripheral Blood Mononuclear Cell Pellets and Plasma from a Single Blood Draw at Clinical Trial Sites for Biomarker Analysis

Published on: March 20, 2021

17.7K

Quantification of biomarker functionality predicts patient outcomes.

Banafshé Larijani1,2,3, James Miles4,5,6,7, Stephen G Ward8

  • 1FASTBASE Solutions S.L., Kabi 612 Scientific and Technology Park of Bizkaia, Derio, 48160, Spain. bl666@bath.ac.uk.

British Journal of Cancer
|March 16, 2021
PubMed
Summary

A new imaging method reveals that low programmed cell death protein 1 (PD-1)/programmed death-ligand 1 (PD-L1) interaction in metastatic non-small cell lung cancer (NSCLC) and melanoma correlates with significantly worse survival outcomes.

More Related Videos

Identification of Disease-related Spatial Covariance Patterns using Neuroimaging Data
14:27

Identification of Disease-related Spatial Covariance Patterns using Neuroimaging Data

Published on: June 26, 2013

16.0K
Predictive Immune Modeling of Solid Tumors
08:50

Predictive Immune Modeling of Solid Tumors

Published on: February 25, 2020

7.3K

Related Experiment Videos

Last Updated: Nov 12, 2025

Preparation of Peripheral Blood Mononuclear Cell Pellets and Plasma from a Single Blood Draw at Clinical Trial Sites for Biomarker Analysis
07:40

Preparation of Peripheral Blood Mononuclear Cell Pellets and Plasma from a Single Blood Draw at Clinical Trial Sites for Biomarker Analysis

Published on: March 20, 2021

17.7K
Identification of Disease-related Spatial Covariance Patterns using Neuroimaging Data
14:27

Identification of Disease-related Spatial Covariance Patterns using Neuroimaging Data

Published on: June 26, 2013

16.0K
Predictive Immune Modeling of Solid Tumors
08:50

Predictive Immune Modeling of Solid Tumors

Published on: February 25, 2020

7.3K

Area of Science:

  • Oncology
  • Molecular Imaging
  • Immunotherapy

Background:

  • The programmed cell death protein 1 (PD-1) and its ligand PD-1 ligand 1 (PD-L1) pathway is a critical regulator of the immune response.
  • Dysregulation of the PD-1/PD-L1 pathway is implicated in tumor immune evasion and progression in various cancers.
  • Quantitative assessment of receptor-ligand interactions can provide prognostic information.

Purpose of the Study:

  • To implement and evaluate the utility of intensity-based Förster Resonance Energy Transfer (iFRET) for quantifying PD-1/PD-L1 interactions in vivo.
  • To determine the prognostic significance of PD-1/PD-L1 interaction levels in patients with metastatic non-small cell lung cancer (NSCLC) and malignant melanoma.

Main Methods:

  • Quantitative molecular imaging using intensity-based Förster Resonance Energy Transfer (iFRET) was employed.
  • iFRET was used to measure the extent of PD-1/PD-L1 receptor-ligand interactions in tumor tissues.
  • Overall survival data was analyzed in relation to the measured levels of PD-1/PD-L1 interaction.

Main Results:

  • Implementation of iFRET enabled quantitative assessment of PD-1/PD-L1 interactions.
  • Patients with metastatic NSCLC and malignant melanoma exhibiting low levels of PD-1/PD-L1 interaction showed significantly worse overall survival.
  • Conversely, patients with high levels of PD-1/PD-L1 interaction demonstrated improved overall survival.

Conclusions:

  • Quantitative molecular imaging of PD-1/PD-L1 interactions using iFRET is feasible and provides prognostic value.
  • Low PD-1/PD-L1 interaction is a significant negative prognostic biomarker in metastatic NSCLC and melanoma.
  • These findings may inform the development of targeted immunotherapies and patient stratification strategies.