Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

7.6K
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
7.6K
Mass Spectrometry: Overview01:19

Mass Spectrometry: Overview

7.5K
Mass spectrometry is an analytical technique used to determine the molecular mass and molecular formula of a compound. The basic principle of mass spectrometry is to generate ions from the analyte molecule and measure these ion abundances against their molecular mass.  One common type of ionization, known as electrospray ionization or EI, bombards the analyte molecules in the gas phase with high-energy electron beams. The electron beams displace an electron from the molecule and leave...
7.5K
High-Resolution Mass Spectrometry (HRMS)01:15

High-Resolution Mass Spectrometry (HRMS)

2.0K
The resolution of a mass spectrometer depends on the efficiency of separating ions with different ion masses. The mass of an atom is approximated to the sum of the masses of protons and neutrons inside, considering the masses of protons and neutrons as equal. However, the masses of the proton (1.6726 × 10−24 g) and neutron (1.6749 × 10−24 g) are not truly equal. There is a minor error in the expression of atomic masses relative to the simplest atom of hydrogen. For...
2.0K
Mass Spectrometers01:16

Mass Spectrometers

7.7K
This lesson details the instrumentation of a mass spectrometer—a physical instrument to perform mass spectrometry on analyte molecules and record the characteristic mass spectra. This is achieved via three chief functions:
7.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Measles vaccination status and evidence of immunity among medical students in Catalonia (Spain), 2023-2025: a cross-sectional study.

BMC medical education·2026
Same author

Modelling Hepatitis B virus related hospital discharges in Spain: ARIMAX based liver disease forecasting tool for hospital workload and mortality progression.

PloS one·2026
Same author

Exploiting androgen receptor agonism as a treatment strategy in estrogen receptor-positive metastatic breast cancer.

NPJ breast cancer·2026
Same author

iDeepLC: Chemical Structure Information Yields Improved Retention Time Prediction of Peptides with Unseen Modifications.

Analytical chemistry·2026
Same author

A silicon microneedle array atmospheric pressure plasma ionization source for real-time trace gas chemical analysis.

Microsystems & nanoengineering·2026
Same author

Serological Mumps Evaluation of Medical Students in Catalonia (Spain): Is a Third Dose of Vaccine Needed?

Journal of medical virology·2026

Related Experiment Video

Updated: Nov 12, 2025

Navigating the Mass Spectrometry-Based Proteomic Data Using Free Computational Tools
07:01

Navigating the Mass Spectrometry-Based Proteomic Data Using Free Computational Tools

Published on: August 19, 2025

392

QCloud2: An Improved Cloud-based Quality-Control System for Mass-Spectrometry-based Proteomics Laboratories.

Roger Olivella1,2, Cristina Chiva1,2, Marc Serret1,2

  • 1Centre de Regulació Genòmica (CRG), Barcelona Institute of Science and Technology (BIST), Dr. Aiguader 88, Barcelona 08003, Spain.

Journal of Proteome Research
|March 16, 2021
PubMed
Summary

QCloud2 enhances proteomics quality control with improved scalability and data visualization. This updated system offers automated data processing and user management for better laboratory performance and research insights.

Keywords:
cloud computingcomputational proteomicsproteomicsquality control

More Related Videos

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

12.3K
Comprehensive Workflow of Mass Spectrometry-based Shotgun Proteomics of Tissue Samples
14:51

Comprehensive Workflow of Mass Spectrometry-based Shotgun Proteomics of Tissue Samples

Published on: November 13, 2021

5.7K

Related Experiment Videos

Last Updated: Nov 12, 2025

Navigating the Mass Spectrometry-Based Proteomic Data Using Free Computational Tools
07:01

Navigating the Mass Spectrometry-Based Proteomic Data Using Free Computational Tools

Published on: August 19, 2025

392
Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

12.3K
Comprehensive Workflow of Mass Spectrometry-based Shotgun Proteomics of Tissue Samples
14:51

Comprehensive Workflow of Mass Spectrometry-based Shotgun Proteomics of Tissue Samples

Published on: November 13, 2021

5.7K

Area of Science:

  • Proteomics
  • Bioinformatics
  • Laboratory Quality Control

Background:

  • QCloud is a cloud-based system designed for daily quality assessment in proteomics laboratories.
  • It offers a user-friendly interface, easy setup, and automated data processing for instrument performance evaluation.
  • QCloud addresses analytical challenges in clinical and translational research by facilitating automated quality control.

Purpose of the Study:

  • To introduce QCloud2, an improved version of the QCloud system.
  • To enhance the scalability and reproducibility of quality-control pipelines.
  • To provide an improved front end for data visualization, user management, and chart annotation.

Main Methods:

  • Development of QCloud2 with enhanced features for quality control in proteomics.
  • Implementation of improved front-end for data visualization and user management.
  • Inclusion of programmatic access and a standalone local version for increased flexibility.

Main Results:

  • QCloud2 offers enhanced scalability and reproducibility in quality-control pipelines.
  • The new version features an improved front end for superior data visualization and user management.
  • Programmatic access and a standalone local version expand the system's utility.

Conclusions:

  • QCloud2 represents a significant advancement in supporting proteomics laboratories with automated quality assessment.
  • The system's enhancements facilitate more robust and flexible quality control for research.
  • QCloud2 is poised to further assist in overcoming analytical challenges in clinical and translational proteomics.