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Updated: Nov 12, 2025

Digital Inline Holographic Microscopy DIHM of Weakly-scattering Subjects
Published on: February 8, 2014
Single-shot higher-order transport-of-intensity quantitative phase imaging based on computer-generated holography
Abstract:
The imaging quality of quantitative phase imaging (QPI) based on the transport of intensity equation (TIE) can be improved using a higher-order approximation for defocused intensity distributions. However, this requires mechanically scanning an image sensor or object along the optical axis, which in turn requires a precisely aligned optical setup. To overcome this problem, a computer-generated hologram (CGH) technique is introduced to TIE-based QPI. A CGH generating defocused point spread function is inserted in the Fourier plane of an object. The CGH acts as a lens and grating with various focal lengths and orientations, allowing multiple defocused intensity distributions to be simultaneously detected on an image sensor plane. The results of a numerical simulation and optical experiment demonstrated the feasibility of the proposed method.
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