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Protein Dynamics in Living Cells

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

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Related Experiment Video

Updated: Nov 12, 2025

TIRFM and pH-sensitive GFP-probes to Evaluate Neurotransmitter Vesicle Dynamics in SH-SY5Y Neuroblastoma Cells: Cell Imaging and Data Analysis

TIRFM and pH-sensitive GFP-probes to Evaluate Neurotransmitter Vesicle Dynamics in SH-SY5Y Neuroblastoma Cells: Cell Imaging and Data Analysis

Published on: January 29, 2015

Methods of measuring presynaptic function with fluorescence probes.

Yeseul Jang¹, Sung Rae Kim¹, Sung Hoon Lee²

¹College of Pharmacy, Chung-Ang University, Seoul, 06974, Republic of Korea.

Applied Microscopy

|March 17, 2021

Summary

This summary is machine-generated.

This study reviews methods for observing synaptic vesicle dynamics, crucial for understanding neurotransmitter release and neuronal function. It highlights techniques for visualizing these small vesicles in living neurons.

Keywords:

Exo- and endocytosis Fluorescence probes Presynaptic terminal Synaptic vesicles

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Measuring Synaptic Vesicle Endocytosis in Cultured Hippocampal Neurons

Measuring Synaptic Vesicle Endocytosis in Cultured Hippocampal Neurons

Published on: September 4, 2017

Area of Science:

Neuroscience
Cell Biology

Background:

Synaptic vesicles are essential for neurotransmitter release and recycling via exocytosis and endocytosis.
Observing synaptic vesicle dynamics (40-50 nm) in living neurons is challenging but vital for understanding neuronal function.
Existing fluorescent labeling methods for synaptic vesicles have limitations.

Purpose of the Study:

To introduce and describe methods for measuring presynaptic activity.
To detail the characteristics, strengths, and drawbacks of various synaptic vesicle labeling techniques.

Main Methods:

Fluorescent labeling of synaptic vesicles.
Measurement of fluorescence intensity changes to confirm exo- and endocytosis.
Review of existing techniques for observing synaptic vesicle dynamics.

Main Results:

Synaptic vesicle exo- and endocytosis can be confirmed using fluorescent labeling and intensity measurements.
Different methods for synaptic vesicle observation possess unique advantages and disadvantages.
The study provides an overview of techniques applicable to presynaptic activity measurement.

Conclusions:

Accurate observation of synaptic vesicle dynamics requires advanced techniques.
Understanding these dynamics is key to deciphering neuronal communication.
The choice of method depends on specific research needs and experimental constraints.