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Updated: Nov 12, 2025
Corneal Donor Tissue Preparation for Descemet's Membrane Endothelial Keratoplasty
Published on: September 17, 2014
The correct graft orientation during descemet membrane endothelial keratoplasty (DMEK) using the "bubble-tap"
Georgios Perdikakis1, Sofia Fili2, Iraklis Vastardis3
1Clinic of Ophthalmology, St. Johannes Hospital, Johannesstrasse 9-17, 44137, Dortmund, Germany. dr.perdi@gmail.com.
Purpose:
To demonstrate a novel, alternative endothelium Descemet membrane layer (EDM) orientation method in Descemet membrane endothelial keratoplasty (DMEK) that does not involve prior ink or trepanation marking of the graft, or intraoperative ocular coherence tomography (OCT) guided graft implantation during surgery, thus preventing the occurrence of an upside-down graft implantation that leads with certainty to primary graft failure.
Methods:
From 2017 to early 2020, 500 eyes underwent DMEK operation using the "bubble-tap" technique first described from Dr. Perdikakis in the department of ophthalmology of St.-Johannes- Hospital in Dortmund in Germany. Primary graft failure and re-bubbling results following "bubble-tap" assisted DMEK are presented.
Results:
Primary graft failure due to upside-down graft implantation was not observed in any patient. Re-bubbling was performed in 4.8% of the eyes. In 1.8% of the cases, a re-DMEK was performed due to a graft failure.
Conclusion:
The "bubble-tap" technique is a novel, reliable and easy to master orientation method of EDM that enables the surgeon to perform DMEK with safety even in complicated cases with poor visibility in the anterior chamber, while it yields superior or equal results in comparison to other graft marking methods or intraoperative OCT-guided graft implantation.
