Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Laser-Emitting Droplet Assay for Enzymatic Evaluation Applications.

ACS nano·2026
Same author

Smartphone-integrated RPA-CRISPR/Cas12a detection system with microneedle sampling for early point-of-care diagnosis of potato late blight.

Biosensors & bioelectronics·2026
Same author

Discovery of fluoxakalner, a novel and preferential Kv7.2/7.3 channel opener for antinociception in mice.

Bioorganic chemistry·2026
Same author

Role of TRPA1 channel activation in exacerbating allergic airway inflammation mediated by 4-Nitrodiphenylamine.

Chemico-biological interactions·2026
Same author

Deterministic radial displacement: modular, reconfigurable, and reusable.

Lab on a chip·2026
Same author

Portable and Point-of-Care Testing Approach for Determining Soil Extracellular Enzyme Activities.

Micromachines·2026
Same journal

Heterojunction Gate-Empowered OPECT Aptasensing: A Valid Protocol for Realizing High Current Gain at Low Electron Donor Dependency.

Analytical chemistry·2026
Same journal

Development of a Tapping-Mode Scanning Probe Electrospray Ionization Platform for High-Sensitivity and Long-Term Stability in Single-Cell Mass Spectrometry Imaging of Tissue.

Analytical chemistry·2026
Same journal

A Solid-State Near-Infrared Fluorescent Probe by a Synergistic Extended Conjugated System for Detecting Cys with Long-Term Imaging in Orthotopic Bladder Cancer.

Analytical chemistry·2026
Same journal

Air-Based Porous Array Dielectric Barrier Discharge Ionization Source for Explosive Trace Detection.

Analytical chemistry·2026
Same journal

PSAQ<sup>+1</sup>: Absolute Protein Quantification Using a <sup>13</sup>C<sub>1</sub>-Labeled Protein Standard, Coisolation of Peptide Pairs and LC-PRM.

Analytical chemistry·2026
Same journal

Heterojunction-Enhanced Interfacial Evanescent-Tunable Fiber Optic Probe for Amplification-free CRISPR/Cas12a-Based Rapid and Ultrasensitive Detection of MPXV.

Analytical chemistry·2026
See all related articles

Related Experiment Video

Updated: Nov 12, 2025

Microfabrication of Implantable Optics Integrated in a Microstructured Imaging Window for Advanced In Vivo Imaging
07:14

Microfabrication of Implantable Optics Integrated in a Microstructured Imaging Window for Advanced In Vivo Imaging

Published on: April 11, 2025

974

Imaging-Based Optofluidic Biolaser Array Encapsulated with Dynamic Living Organisms.

Xuerui Gong1, Shilun Feng2, Zhen Qiao1

  • 1School of Electrical and Electronics Engineering, Nanyang Technological University, 50 Nanyang Ave., 639798, Singapore.

Analytical Chemistry
|March 18, 2021
PubMed
Summary
This summary is machine-generated.

This study introduces image-based lasing analysis using microdroplet lasers with living Escherichia coli. This method offers a more sensitive and high-throughput approach for monitoring biological changes compared to traditional fluorescence analysis.

More Related Videos

Laser-Induced Fluorescence Emission L.I.F.E. as Novel Non-Invasive Tool for In-Situ Measurements of Biomarkers in Cryospheric Habitats
13:38

Laser-Induced Fluorescence Emission L.I.F.E. as Novel Non-Invasive Tool for In-Situ Measurements of Biomarkers in Cryospheric Habitats

Published on: October 26, 2019

8.2K
Design and Implementation of an Automated Illuminating, Culturing, and Sampling System for Microbial Optogenetic Applications
11:13

Design and Implementation of an Automated Illuminating, Culturing, and Sampling System for Microbial Optogenetic Applications

Published on: February 19, 2017

9.9K

Related Experiment Videos

Last Updated: Nov 12, 2025

Microfabrication of Implantable Optics Integrated in a Microstructured Imaging Window for Advanced In Vivo Imaging
07:14

Microfabrication of Implantable Optics Integrated in a Microstructured Imaging Window for Advanced In Vivo Imaging

Published on: April 11, 2025

974
Laser-Induced Fluorescence Emission L.I.F.E. as Novel Non-Invasive Tool for In-Situ Measurements of Biomarkers in Cryospheric Habitats
13:38

Laser-Induced Fluorescence Emission L.I.F.E. as Novel Non-Invasive Tool for In-Situ Measurements of Biomarkers in Cryospheric Habitats

Published on: October 26, 2019

8.2K
Design and Implementation of an Automated Illuminating, Culturing, and Sampling System for Microbial Optogenetic Applications
11:13

Design and Implementation of an Automated Illuminating, Culturing, and Sampling System for Microbial Optogenetic Applications

Published on: February 19, 2017

9.9K

Area of Science:

  • Optofluidics
  • Biophotonics
  • Microfluidics

Background:

  • Optofluidic biolasers offer strong light-matter interactions and miniaturization for biomedical analysis.
  • Current biolasers often require single-cavity spectral analysis, limiting high-throughput applications.
  • Monitoring dynamic biological processes requires sensitive and efficient analytical methods.

Purpose of the Study:

  • To develop a high-throughput, image-based analysis method for living organisms using optofluidic biolasers.
  • To demonstrate the sensitivity of laser emission imaging for detecting dynamic biological changes.
  • To compare the efficacy of laser imaging versus fluorescence analysis for quantifying biological responses.

Main Methods:

  • Fabrication of a microdroplet laser array encapsulated with living Escherichia coli on reflective mirrors.
  • Utilizing laser emission images from whispering-gallery modes for analysis.
  • Quantifying integrated pixel intensity of lasing images to reflect biological dynamics.
  • Comparing dynamic interactions of E. coli with antibiotics using both laser emission and fluorescence imaging.

Main Results:

  • Laser emission imaging successfully reflected dynamic changes in living Escherichia coli.
  • Image-based lasing analysis demonstrated a significant increase in integrated pixel intensity (2 orders of magnitude).
  • The amplification during laser generation allowed quantification of subtle biological changes.

Conclusions:

  • Image-based lasing analysis is more sensitive to dynamic biological changes than fluorescence analysis.
  • This approach enables high-throughput, on-chip laser analysis of living organisms.
  • Optofluidic biolasers provide a powerful platform for sensitive biomedical monitoring.