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Phage Phenomics: Physiological Approaches to Characterize Novel Viral Proteins
Published on: June 11, 2015
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Complete Genome Sequence of Mycobacteriophage Fulbright
Hari Kotturi1, Umar Sahi2, Cameron Kedy2
1Department of Biology, University of Central Oklahoma, Edmond, Oklahoma, USA hkotturi@uco.edu.
Microbiology Resource Announcements
|March 19, 2021
Summary
Mycobacteriophage Fulbright was isolated from Oklahoma soil. Its genome analysis reveals 70 open reading frames, aiding in understanding mycobacterial phages.
Area of Science:
- Microbiology
- Virology
- Genomics
Background:
- Mycobacteriophages are viruses that infect Mycobacterium bacteria.
- Understanding phage diversity is crucial for developing novel therapeutic strategies.
- Mycobacterium smegmatis is a commonly used host for phage isolation and characterization.
Purpose of the Study:
- To isolate and characterize a novel mycobacteriophage from soil.
- To determine the genomic features of the isolated phage.
- To classify the phage within existing mycobacteriophage clusters.
Main Methods:
- Isolation of mycobacteriophage Fulbright from soil using Mycobacterium smegmatis mc2115.
- Whole-genome sequencing and analysis of the phage.
- Bioinformatic analysis to identify open reading frames (ORFs) and compare with known phages.
Main Results:
- Isolation of a viable mycobacteriophage, named Fulbright.
- The genome of phage Fulbright is 42,396 base pairs long.
- The genome contains 70 open reading frames (ORFs), with 33 having predicted functions and 37 encoding hypothetical proteins.
- Phage Fulbright belongs to cluster N and shows 99% nucleotide identity to mycobacteriophage Phloss.
Conclusions:
- Mycobacteriophage Fulbright represents a new addition to the known mycobacteriophage repertoire.
- Genomic analysis provides insights into the genetic makeup and potential functions of phage Fulbright.
- The high similarity to Phloss suggests a close evolutionary relationship within cluster N.
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