Related Experiment Video
Updated: Aug 22, 2025
13:24
An Optimized Enrichment Technique for the Isolation of Arthrobacter Bacteriophage Species from Soil Sample Isolates
Published on: April 9, 2015
19.7K
Complete Genome Sequence of Arthrobacter Phage ScienceWizSam
Samantha Coplen1, Charmaine Lopez-Davis1, Hari Kotturi1
1Department of Biology, University of Central Oklahoma, Edmond, Oklahoma, USA.
Microbiology Resource Announcements
|November 7, 2022
Summary
Researchers isolated the ScienceWizSam phage from soil bacteria. This phage, belonging to subcluster AU1, has a genome with 96 genes transcribed in one direction.
Area of Science:
- Microbiology
- Virology
- Genomics
Background:
- Bacteriophages are viruses that infect bacteria.
- The characterization of novel phages is crucial for understanding microbial ecosystems and developing phage-based applications.
Purpose of the Study:
- To isolate and characterize a novel bacteriophage, ScienceWizSam.
- To determine the genomic features and taxonomic placement of ScienceWizSam.
Main Methods:
- Isolation of bacteriophage ScienceWizSam from soil using Arthrobacter sp. strain ATCC 21022.
- Genomic sequencing and analysis to identify open reading frames (ORFs) and transcriptional direction.
- Comparative genomic analysis for taxonomic classification.
Main Results:
- The bacteriophage ScienceWizSam was successfully isolated.
- The ScienceWizSam genome comprises 58,217 base pairs with 96 open reading frames (ORFs).
- All identified ORFs are transcribed in a rightward direction, and the phage is assigned to subcluster AU1 based on gene content.
Conclusions:
- ScienceWizSam represents a newly characterized bacteriophage with specific genomic features.
- Its classification within subcluster AU1 provides insights into phage diversity and evolution.
- Further studies on ScienceWizSam could elucidate its biological role and potential applications.
Related Concept Videos
01:26DNA Bacteriophages
99
Bacteriophages, or phages, are viruses that specifically infect bacteria, utilizing their genetic material to hijack host cellular machinery for replication. DNA bacteriophages employ single-stranded DNA (ssDNA) or double-stranded DNA (dsDNA) genomes. These phages exhibit diverse replication strategies and host interactions, influencing their ecological roles and applications in biotechnology and medicine.ssDNA BacteriophagesssDNA phages, with their small genomes, utilize unique strategies to...
99
01:29Viruses of Archaea
62
Archaeal viruses play a crucial role in the ecosystems of extremophilic archaea, particularly those belonging to the phyla Euryarchaeota and Crenarchaeota. By shaping host evolution and facilitating gene transfer, these viruses influence microbial communities and contribute to genetic diversity in extreme environments. The archaea they infect thrive in acidic hot springs and hydrothermal vents characterized by high temperatures and low pH. Archaeal viruses exhibit remarkable structural...
62
00:46Genomic DNA in Prokaryotes
44.3K
The genome of most prokaryotic organisms consists of double-stranded DNA organized into one circular chromosome in a region of cytoplasm called the nucleoid. The chromosome is tightly wound, or supercoiled, for efficient storage. Prokaryotes also contain other circular pieces of DNA called plasmids. These plasmids are smaller than the chromosome and often carry genes that confer adaptive functions, such as antibiotic resistance.
Genomic Diversity in Bacteria
Although bacterial genomes are much...
Genomic Diversity in Bacteria
Although bacterial genomes are much...
44.3K
01:23The Antiviral System of Bacteria and Archaea: CRISPR
95
CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats is a adaptive immune system found in bacteria and archaea that protects against viral infections. This system enables prokaryotic cells to identify, remember, and neutralize foreign genetic elements, primarily bacteriophages, by storing fragments of the invader’s DNA as a genetic memory.The CRISPR immune response begins during an initial infection. Cas (CRISPR-associated) proteins play a central role in this...
95
01:30Lytic Cycle of Bacteriophages
71.5K
Bacteriophages, also known as phages, are specialized viruses that infect bacteria. A key characteristic of phages is their distinctive “head-tail” morphology. A phage begins the infection process (i.e., lytic cycle) by attaching to the outside of a bacterial cell. Attachment is accomplished via proteins in the phage tail that bind to specific receptor proteins on the outer surface of the bacterium. The tail injects the phage’s DNA genome into the bacterial cytoplasm. In the...
71.5K
01:20Viral Replication: Lytic Cycle
153
Bacteriophages, or phages, are viruses that specifically infect bacteria. Among them, T-even bacteriophages, such as T4, exhibit a well-characterized lytic replication cycle in Escherichia coli (E. coli). This process ensures the rapid proliferation of the virus while ultimately leading to the destruction of the bacterial host.Attachment and DNA InjectionThe infection process begins with the recognition and binding of the T4 phage to the E. coli cell surface. Tail fibers of the phage...
153