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Long non-coding RNA lnc_3712 impedes nuclear reprogramming via repressing Kdm5b.
Mingtian Deng1, Yongjie Wan1, Baobao Chen2
1Jiangsu Livestock Embryo Engineering Laboratory, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
Molecular Therapy. Nucleic Acids
|March 19, 2021
Summary
Long non-coding RNA (lncRNA) lnc_3712 represses Kdm5b, improving goat somatic cell nuclear transfer (SCNT) embryo development by removing epigenetic barriers. Knockdown of lnc_3712 enhances reprogramming and embryonic gene expression.
Area of Science:
- Epigenetics
- Developmental Biology
- Genomics
Background:
- Long non-coding RNAs (lncRNAs) influence chromosome structure and early development.
- The function of lncRNAs in somatic cell nuclear transfer (SCNT) reprogramming is not well understood.
Purpose of the Study:
- To investigate the role of lncRNAs in goat SCNT reprogramming.
- To identify key lncRNAs involved in improving SCNT embryo development.
Main Methods:
- Identified upregulated lncRNAs in goat SCNT embryos.
- Investigated the epigenetic barrier H3K4me3 and the role of Kdm5b.
- Utilized lncRNA knockdown and mRNA injection techniques.
- Analyzed embryonic gene expression and developmental rates.
Main Results:
- 114 lncRNAs were upregulated in 8-cell SCNT embryos.
- H3K4me3 acts as an epigenetic barrier; Kdm5b injection improved SCNT development.
- Knockdown of lnc_3712 increased Kdm5b, leading to H3K4me3 demethylation and improved SCNT embryo development.
- lnc_3712 knockdown restored embryonic gene expression similar to in vitro fertilization embryos.
Conclusions:
- lnc_3712 plays a critical role in regulating goat SCNT embryo development by repressing Kdm5b.
- This study advances understanding of lncRNA functions in nuclear reprogramming.
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